Detailed information of TA system TA045329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 123456..123832
• Replicon: plasmid p3PCN033
• Accession: NZ_CP006635
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: PPECC33_RS25325
• Protein ID: WP_000969988.1
• Coordinates: 123456..123638 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: PPECC33_RS25330
• Protein ID: WP_001324224.1
• Coordinates: 123635..123832 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS25310	121455..122489	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
PPECC33_RS25320	123049..123357	-	309	WP_000377483.1	hypothetical protein	-
PPECC33_RS25325	123456..123638	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPECC33_RS25330	123635..123832	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
PPECC33_RS25335	124514..125788	+	1275	WP_000489609.1	HlyD family secretion protein	-
PPECC33_RS25340	125763..127877	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
PPECC33_RS25345	128047..128358	-	312	WP_001259758.1	colicin V	-
PPECC33_RS28750	128336..128572	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD / oqxA / oqxB / blaTEM-1B / tet(B)	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..161511	161511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T45329 WP_000969988.1 NZ_CP006635:c123638-123456 [Escherichia coli PCN033]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T45329 NZ_CP006635:c123638-123456 [Escherichia coli PCN033]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT45329 WP_001324224.1 NZ_CP006635:c123832-123635 [Escherichia coli PCN033]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT45329 NZ_CP006635:c123832-123635 [Escherichia coli PCN033]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3