Detailed information of TA system TA045324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 28292..28917
• Replicon: plasmid p3PCN033
• Accession: NZ_CP006635
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PPECC33_RS24755
• Protein ID: WP_000911313.1
• Coordinates: 28519..28917 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: PPECC33_RS24750
• Protein ID: WP_000450520.1
• Coordinates: 28292..28519 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS24750	28292..28519	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
PPECC33_RS24755	28519..28917	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PPECC33_RS24760	28926..31079	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
PPECC33_RS24765	31332..32063	-	732	WP_023565183.1	complement resistance protein TraT	-
PPECC33_RS24770	32095..32592	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD / oqxA / oqxB / blaTEM-1B / tet(B)	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..161511	161511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T45324 WP_000911313.1 NZ_CP006635:28519-28917 [Escherichia coli PCN033]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T45324 NZ_CP006635:28519-28917 [Escherichia coli PCN033]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT45324 WP_000450520.1 NZ_CP006635:28292-28519 [Escherichia coli PCN033]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT45324 NZ_CP006635:28292-28519 [Escherichia coli PCN033]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2