Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4849264..4850099 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name yeeV Uniprot ID V0Z9U3
Locus tag PPECC33_RS23835 Protein ID WP_000854750.1
Coordinates 4849722..4850099 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2J7L0S7
Locus tag PPECC33_RS23830 Protein ID WP_001278231.1
Coordinates 4849264..4849632 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS23790 4844489..4845271 - 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
PPECC33_RS23795 4845268..4846290 - 1023 WP_001375934.1 IS21-like element IS100 family transposase -
PPECC33_RS29000 4846378..4846569 + 192 Protein_4507 hypothetical protein -
PPECC33_RS23800 4846575..4846808 + 234 WP_001278285.1 DUF905 family protein -
PPECC33_RS23805 4846907..4847724 + 818 Protein_4509 DUF945 domain-containing protein -
PPECC33_RS23810 4847816..4848301 + 486 WP_047938471.1 antirestriction protein -
PPECC33_RS23815 4848317..4848793 + 477 WP_001367707.1 RadC family protein -
PPECC33_RS23820 4848880..4849101 + 222 WP_000691819.1 DUF987 domain-containing protein -
PPECC33_RS23830 4849264..4849632 + 369 WP_001278231.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
PPECC33_RS23835 4849722..4850099 + 378 WP_000854750.1 TA system toxin CbtA family protein Toxin
PPECC33_RS23840 4850096..4850584 + 489 WP_000761685.1 hypothetical protein -
PPECC33_RS28430 4850596..4850676 + 81 Protein_4516 DUF957 domain-containing protein -
PPECC33_RS23850 4850767..4851995 + 1229 WP_164473239.1 IS3-like element IS2 family transposase -
PPECC33_RS23855 4851980..4852129 + 150 WP_023148250.1 DUF957 domain-containing protein -
PPECC33_RS29005 4852214..4852360 + 147 WP_001290178.1 hypothetical protein -
PPECC33_RS23860 4852963..4853742 + 780 WP_001088804.1 HNH endonuclease -
PPECC33_RS23865 4853811..4854188 + 378 WP_000784770.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 4808592..4878242 69650
- inside IScluster/Tn - - 4844489..4851995 7506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13919.90 Da        Isoelectric Point: 8.2905

>T45319 WP_000854750.1 NZ_CP006632:4849722-4850099 [Escherichia coli PCN033]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T45319 NZ_CP006632:4849722-4850099 [Escherichia coli PCN033]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT45319 WP_001278231.1 NZ_CP006632:4849264-4849632 [Escherichia coli PCN033]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLEIMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT45319 NZ_CP006632:4849264-4849632 [Escherichia coli PCN033]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAATCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0Z9U3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J7L0S7

References