Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4766692..4767214 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag PPECC33_RS23425 Protein ID WP_001105433.1
Coordinates 4766924..4767214 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag PPECC33_RS23420 Protein ID WP_000212715.1
Coordinates 4766692..4766934 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS28400 4762486..4763834 + 1349 WP_100190663.1 IS3 family transposase -
PPECC33_RS23405 4763934..4764485 - 552 WP_000166295.1 ribosome-associated protein -
PPECC33_RS23410 4764579..4765931 + 1353 WP_001162171.1 metalloprotease PmbA -
PPECC33_RS23415 4766114..4766500 + 387 WP_001232246.1 cytochrome b562 -
PPECC33_RS23420 4766692..4766934 + 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
PPECC33_RS23425 4766924..4767214 + 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PPECC33_RS23430 4767215..4767679 - 465 WP_001106233.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
PPECC33_RS23435 4767837..4769975 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
PPECC33_RS23445 4770369..4772024 - 1656 WP_001376150.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T45318 WP_001105433.1 NZ_CP006632:4766924-4767214 [Escherichia coli PCN033]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T45318 NZ_CP006632:4766924-4767214 [Escherichia coli PCN033]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT45318 WP_000212715.1 NZ_CP006632:4766692-4766934 [Escherichia coli PCN033]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT45318 NZ_CP006632:4766692-4766934 [Escherichia coli PCN033]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References