Detailed information of TA system TA045317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4753644..4754239
• Replicon: chromosome
• Accession: NZ_CP006632
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: PPECC33_RS23350
• Protein ID: WP_000239581.1
• Coordinates: 4753889..4754239 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: PPECC33_RS23345
• Protein ID: WP_001223213.1
• Coordinates: 4753644..4753895 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS23335	4749309..4753088	+	3780	WP_000060864.1	autotransporter assembly complex protein TamB	-
PPECC33_RS23340	4753091..4753432	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
PPECC33_RS23345	4753644..4753895	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
PPECC33_RS23350	4753889..4754239	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
PPECC33_RS23355	4754318..4754848	-	531	WP_000055075.1	inorganic diphosphatase	-
PPECC33_RS23360	4755158..4756114	+	957	WP_000265935.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
PPECC33_RS23365	4756247..4757749	+	1503	WP_001095665.1	sugar ABC transporter ATP-binding protein	-
PPECC33_RS23370	4757763..4758785	+	1023	WP_001376149.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T45317 WP_000239581.1 NZ_CP006632:4753889-4754239 [Escherichia coli PCN033]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T45317 NZ_CP006632:4753889-4754239 [Escherichia coli PCN033]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT45317 WP_001223213.1 NZ_CP006632:4753644-4753895 [Escherichia coli PCN033]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT45317 NZ_CP006632:4753644-4753895 [Escherichia coli PCN033]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7