Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4651145..4651642 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PPECC33_RS22830 Protein ID WP_001173343.1
Coordinates 4651469..4651642 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag PPECC33_RS22825 Protein ID WP_001560287.1
Coordinates 4651145..4651441 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS22815 4646502..4650431 + 3930 WP_001080130.1 autotransporter outer membrane beta-barrel domain-containing protein -
PPECC33_RS22820 4650644..4650916 + 273 WP_000405647.1 N-acetyltransferase -
PPECC33_RS22825 4651145..4651441 + 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PPECC33_RS22830 4651469..4651642 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PPECC33_RS22835 4651761..4653278 - 1518 WP_000003806.1 lysine--tRNA ligase -
PPECC33_RS22840 4653515..4654972 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4637967..4660496 22529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T45316 WP_001173343.1 NZ_CP006632:4651469-4651642 [Escherichia coli PCN033]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T45316 NZ_CP006632:4651469-4651642 [Escherichia coli PCN033]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCAGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT45316 WP_001560287.1 NZ_CP006632:4651145-4651441 [Escherichia coli PCN033]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT45316 NZ_CP006632:4651145-4651441 [Escherichia coli PCN033]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References