Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3961721..3962333 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag PPECC33_RS19580 Protein ID WP_000833473.1
Coordinates 3962148..3962333 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A5F1W1Z0
Locus tag PPECC33_RS19575 Protein ID WP_000499743.1
Coordinates 3961721..3962131 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS19560 3957233..3958048 - 816 WP_000891819.1 AraC family transcriptional regulator -
PPECC33_RS19565 3958274..3959674 + 1401 WP_000204821.1 MFS transporter -
PPECC33_RS19570 3959685..3961649 + 1965 WP_001026888.1 glycoside hydrolase family 127 protein -
PPECC33_RS19575 3961721..3962131 - 411 WP_000499743.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PPECC33_RS19580 3962148..3962333 - 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
PPECC33_RS19590 3962799..3963878 + 1080 WP_000061476.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
PPECC33_RS19595 3963919..3965457 - 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
PPECC33_RS19600 3965658..3966809 - 1152 WP_000741506.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T45315 WP_000833473.1 NZ_CP006632:c3962333-3962148 [Escherichia coli PCN033]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T45315 NZ_CP006632:c3962333-3962148 [Escherichia coli PCN033]
GTGAAAAGTGCAGACGTGATCGCTATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15213.09 Da        Isoelectric Point: 4.5486

>AT45315 WP_000499743.1 NZ_CP006632:c3962131-3961721 [Escherichia coli PCN033]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGA

Download         Length: 411 bp

>AT45315 NZ_CP006632:c3962131-3961721 [Escherichia coli PCN033]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTTGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1W1Z0

References