Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3823697..3824328 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name hicA Uniprot ID A0A792T3R5
Locus tag PPECC33_RS18950 Protein ID WP_001260302.1
Coordinates 3824053..3824328 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID L4IZA1
Locus tag PPECC33_RS18945 Protein ID WP_000593557.1
Coordinates 3823697..3824056 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS18920 3819829..3820773 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
PPECC33_RS18925 3820770..3821603 + 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
PPECC33_RS18930 3821603..3822367 + 765 WP_001136200.1 nickel import ATP-binding protein NikD -
PPECC33_RS18935 3822364..3823170 + 807 WP_000173688.1 nickel import ATP-binding protein NikE -
PPECC33_RS18940 3823176..3823577 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
PPECC33_RS18945 3823697..3824056 - 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PPECC33_RS18950 3824053..3824328 - 276 WP_001260302.1 type II toxin-antitoxin system HicA family toxin Toxin
PPECC33_RS18955 3824401..3825525 - 1125 WP_001366710.1 ABC transporter permease -
PPECC33_RS18960 3825525..3828260 - 2736 WP_000149100.1 ribosome-associated ATPase/putative transporter RbbA -
PPECC33_RS18965 3828257..3829324 - 1068 WP_000361507.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10177.85 Da        Isoelectric Point: 10.6128

>T45314 WP_001260302.1 NZ_CP006632:c3824328-3824053 [Escherichia coli PCN033]
MRTQVQALRKKQKNTLDQIVKSHVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T45314 NZ_CP006632:c3824328-3824053 [Escherichia coli PCN033]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCATGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13389.28 Da        Isoelectric Point: 5.1987

>AT45314 WP_000593557.1 NZ_CP006632:c3824056-3823697 [Escherichia coli PCN033]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT45314 NZ_CP006632:c3824056-3823697 [Escherichia coli PCN033]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACT
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A792T3R5

Antitoxin

Source ID Structure
AlphaFold DB L4IZA1

References