Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3188079..3188733 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag PPECC33_RS15670 Protein ID WP_000244781.1
Coordinates 3188079..3188486 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PPECC33_RS15675 Protein ID WP_000354046.1
Coordinates 3188467..3188733 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS15650 3184036..3185769 - 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -
PPECC33_RS15655 3185775..3186485 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PPECC33_RS15660 3186510..3187406 - 897 WP_000806651.1 site-specific tyrosine recombinase XerD -
PPECC33_RS15665 3187518..3188039 + 522 WP_001055869.1 flavodoxin FldB -
PPECC33_RS15670 3188079..3188486 - 408 WP_000244781.1 protein YgfX Toxin
PPECC33_RS15675 3188467..3188733 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PPECC33_RS15680 3188976..3189956 + 981 WP_000886074.1 tRNA-modifying protein YgfZ -
PPECC33_RS15685 3190033..3190692 - 660 WP_000250268.1 hemolysin III family protein -
PPECC33_RS15690 3190856..3191167 - 312 WP_001182963.1 N(4)-acetylcytidine aminohydrolase -
PPECC33_RS15695 3191212..3192645 + 1434 WP_024177858.1 6-phospho-beta-glucosidase BglA -
PPECC33_RS15700 3192707..3193450 - 744 WP_001366570.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T45312 WP_000244781.1 NZ_CP006632:c3188486-3188079 [Escherichia coli PCN033]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T45312 NZ_CP006632:c3188486-3188079 [Escherichia coli PCN033]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT45312 WP_000354046.1 NZ_CP006632:c3188733-3188467 [Escherichia coli PCN033]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT45312 NZ_CP006632:c3188733-3188467 [Escherichia coli PCN033]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References