Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1595024..1595662 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name hicA Uniprot ID A0A792T4N9
Locus tag PPECC33_RS07825 Protein ID WP_000813798.1
Coordinates 1595024..1595200 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag PPECC33_RS07830 Protein ID WP_001270286.1
Coordinates 1595246..1595662 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS07805 1590646..1591857 - 1212 WP_071526537.1 BenE family transporter YdcO -
PPECC33_RS07810 1591910..1592446 + 537 WP_000429508.1 DNA-binding transcriptional regulator SutR -
PPECC33_RS07815 1592519..1594480 + 1962 WP_024177865.1 23S rRNA 5-hydroxycytidine C2501 synthase -
PPECC33_RS07820 1594572..1594802 - 231 WP_000494241.1 YncJ family protein -
PPECC33_RS07825 1595024..1595200 + 177 WP_000813798.1 hypothetical protein Toxin
PPECC33_RS07830 1595246..1595662 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
PPECC33_RS07835 1595741..1597147 + 1407 WP_000760631.1 PLP-dependent aminotransferase family protein -
PPECC33_RS07840 1597392..1598537 + 1146 WP_000047443.1 ABC transporter substrate-binding protein -
PPECC33_RS07845 1598555..1599568 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
PPECC33_RS07850 1599569..1600510 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6635.66 Da        Isoelectric Point: 10.9293

>T45302 WP_000813798.1 NZ_CP006632:1595024-1595200 [Escherichia coli PCN033]
VKQSEFRRWLESQGVDVANGSNHLKLSFHGRRSVMPRLPSEEIKEPLHKAILKQLGLS

Download         Length: 177 bp

>T45302 NZ_CP006632:1595024-1595200 [Escherichia coli PCN033]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
TTTTCACGGGAGGCGCAGTGTCATGCCGCGTCTCCCCAGCGAAGAGATTAAAGAACCCTTGCATAAAGCTATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT45302 WP_001270286.1 NZ_CP006632:1595246-1595662 [Escherichia coli PCN033]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT45302 NZ_CP006632:1595246-1595662 [Escherichia coli PCN033]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A792T4N9

Antitoxin

Source ID Structure

References