Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 539128..539746 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag PPECC33_RS02560 Protein ID WP_001291435.1
Coordinates 539128..539346 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag PPECC33_RS02565 Protein ID WP_000344800.1
Coordinates 539372..539746 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS02530 534417..534989 + 573 WP_000779829.1 YbaY family lipoprotein -
PPECC33_RS02535 535020..535331 - 312 WP_000409911.1 MGMT family protein -
PPECC33_RS02540 535710..536063 + 354 WP_000878139.1 DUF1428 family protein -
PPECC33_RS02545 536105..537655 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
PPECC33_RS02550 537819..538289 - 471 WP_000136192.1 YlaC family protein -
PPECC33_RS02555 538405..538956 - 552 WP_000102564.1 maltose O-acetyltransferase -
PPECC33_RS02560 539128..539346 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
PPECC33_RS02565 539372..539746 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
PPECC33_RS02570 540292..543441 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
PPECC33_RS02575 543464..544657 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45297 WP_001291435.1 NZ_CP006632:c539346-539128 [Escherichia coli PCN033]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T45297 NZ_CP006632:c539346-539128 [Escherichia coli PCN033]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT45297 WP_000344800.1 NZ_CP006632:c539746-539372 [Escherichia coli PCN033]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT45297 NZ_CP006632:c539746-539372 [Escherichia coli PCN033]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References