Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56095..56645 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7A0MYA0
Locus tag PPECC33_RS00285 Protein ID WP_001160967.1
Coordinates 56331..56645 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag PPECC33_RS00280 Protein ID WP_000125566.1
Coordinates 56095..56328 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS00255 51168..51455 + 288 WP_000203740.1 ferredoxin-like protein FixX -
PPECC33_RS00260 51514..52845 + 1332 WP_001183197.1 MFS transporter -
PPECC33_RS00265 52953..53483 + 531 WP_000600723.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
PPECC33_RS00270 53476..55338 + 1863 WP_000377111.1 glutathione-regulated potassium-efflux system protein KefC -
PPECC33_RS00275 55530..56009 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
PPECC33_RS00280 56095..56328 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
PPECC33_RS00285 56331..56645 + 315 WP_001160967.1 CcdB family protein Toxin
PPECC33_RS00290 56642..57490 - 849 WP_000257196.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
PPECC33_RS00295 57497..57874 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
PPECC33_RS00300 57877..58698 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
PPECC33_RS00305 58695..59684 - 990 WP_000241266.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
PPECC33_RS00310 59684..60970 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11672.68 Da        Isoelectric Point: 8.0406

>T45295 WP_001160967.1 NZ_CP006632:56331-56645 [Escherichia coli PCN033]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T45295 NZ_CP006632:56331-56645 [Escherichia coli PCN033]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGGGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT45295 WP_000125566.1 NZ_CP006632:56095-56328 [Escherichia coli PCN033]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT45295 NZ_CP006632:56095-56328 [Escherichia coli PCN033]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7A0MYA0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References