Detailed information of TA system TA045294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2681745..2682853
• Replicon: chromosome
• Accession: NZ_CP006630
• Organism: Staphylococcus aureus subsp. aureus SA268

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: SA268_RS13630
• Protein ID: WP_000233000.1
• Coordinates: 2681745..2682614 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: SA268_RS13635
• Protein ID: WP_000205227.1
• Coordinates: 2682629..2682853 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA268_RS13600	2677238..2677975	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
SA268_RS13605	2678100..2678183	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
SA268_RS15135	2678232..2678315	-	84	Protein_2582	peptide-binding protein	-
SA268_RS13610	2678725..2679519	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
SA268_RS15140	2679612..2680092	-	481	Protein_2584	GNAT family N-acetyltransferase	-
SA268_RS13620	2680089..2680997	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
SA268_RS13625	2681030..2681764	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
SA268_RS13630	2681745..2682614	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
SA268_RS13635	2682629..2682853	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
SA268_RS13640	2682996..2684558	-	1563	WP_000136908.1	recombinase family protein	-
SA268_RS13645	2684560..2684976	-	417	WP_000323438.1	recombinase	-
SA268_RS13650	2684977..2685273	-	297	WP_002360685.1	hypothetical protein	-
SA268_RS13660	2685785..2686462	-	678	Protein_2592	DNA topoisomerase	-
SA268_RS13665	2686478..2687080	-	603	WP_001062586.1	recombinase family protein	-
SA268_RS15440	2687094..2687264	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	erm(B) / aph(3')-III / ant(6)-Ia	-	2677102..2689688	12586
	-	flank	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia	-	2672322..2680997	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T45294 WP_000233000.1 NZ_CP006630:c2682614-2681745 [Staphylococcus aureus subsp. aureus SA268]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T45294 NZ_CP006630:c2682614-2681745 [Staphylococcus aureus subsp. aureus SA268]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT45294 WP_000205227.1 NZ_CP006630:c2682853-2682629 [Staphylococcus aureus subsp. aureus SA268]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT45294 NZ_CP006630:c2682853-2682629 [Staphylococcus aureus subsp. aureus SA268]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1