Detailed information of TA system TA045288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2119052..2119581
• Replicon: chromosome
• Accession: NZ_CP006630
• Organism: Staphylococcus aureus subsp. aureus SA268

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SA268_RS10700
• Protein ID: WP_000621175.1
• Coordinates: 2119052..2119414 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SA268_RS10705
• Protein ID: WP_000948331.1
• Coordinates: 2119411..2119581 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA268_RS10675	2116031..2116801	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SA268_RS10680	2116776..2117255	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SA268_RS10685	2117257..2117583	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SA268_RS10690	2117701..2118702	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SA268_RS10700	2119052..2119414	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SA268_RS10705	2119411..2119581	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SA268_RS10710	2119666..2120814	-	1149	WP_001280702.1	alanine racemase	-
SA268_RS10715	2120880..2121239	-	360	WP_000581194.1	holo-ACP synthase	-
SA268_RS10720	2121243..2121734	-	492	WP_001205915.1	PH domain-containing protein	-
SA268_RS10725	2121721..2123304	-	1584	WP_001294642.1	PH domain-containing protein	-
SA268_RS10730	2123297..2123776	-	480	WP_001287083.1	hypothetical protein	-
SA268_RS10735	2123985..2124545	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T45288 WP_000621175.1 NZ_CP006630:c2119414-2119052 [Staphylococcus aureus subsp. aureus SA268]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T45288 NZ_CP006630:c2119414-2119052 [Staphylococcus aureus subsp. aureus SA268]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT45288 WP_000948331.1 NZ_CP006630:c2119581-2119411 [Staphylococcus aureus subsp. aureus SA268]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT45288 NZ_CP006630:c2119581-2119411 [Staphylococcus aureus subsp. aureus SA268]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1