Detailed information of TA system TA045278

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4268736..4269481
• Replicon: chromosome
• Accession: NZ_CP006580
• Organism: Enterobacter ludwigii strain P101

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: M942_RS20745
• Protein ID: WP_025205053.1
• Coordinates: 4268736..4269104 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: M942_RS20750
• Protein ID: WP_025205055.1
• Coordinates: 4269125..4269481 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M942_RS20725	4264668..4265432	+	765	WP_025205044.1	lectin	-
M942_RS20730	4265496..4266902	+	1407	WP_025205046.1	carbohydrate binding domain-containing protein	-
M942_RS20735	4267087..4267779	-	693	WP_025205048.1	hypothetical protein	-
M942_RS20740	4267888..4268529	+	642	WP_025205050.1	TetR/AcrR family transcriptional regulator	-
M942_RS20745	4268736..4269104	-	369	WP_025205053.1	TA system toxin CbtA family protein	Toxin
M942_RS20750	4269125..4269481	-	357	WP_025205055.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
M942_RS20755	4269507..4269728	-	222	WP_025205057.1	DUF987 domain-containing protein	-
M942_RS20760	4269725..4270267	-	543	WP_025205059.1	DNA repair protein RadC	-
M942_RS20765	4270280..4270723	-	444	WP_025205062.1	antirestriction protein	-
M942_RS20770	4270754..4271575	-	822	WP_025205063.1	DUF945 domain-containing protein	-
M942_RS20775	4271696..4272169	-	474	WP_025205064.1	hypothetical protein	-
M942_RS20780	4272241..4272693	-	453	WP_025205065.1	hypothetical protein	-
M942_RS20785	4272729..4273445	-	717	WP_025205066.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4267888..4300058	32170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13762.92 Da
• Isoelectric Point: 7.6697

>T45278 WP_025205053.1 NZ_CP006580:c4269104-4268736 [Enterobacter ludwigii]
MKNLPATTSRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAVNFLVEKYELVRIDRTGF
TLQEQAPYLTATDILQARKACGLMSRCSYREVSNIVFSRSRL

Nucleotide

• Length: 369 bp

>T45278 NZ_CP006580:c4269104-4268736 [Enterobacter ludwigii]
ATGAAAAATCTACCTGCTACAACTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTACTTAC
ACGGCTGCTGGAACAGCATTATGGTCTGACGCTAAACGATACACCTTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTGGAAAAGTATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTTACAGGAACAAGCGCCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTTTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13135.99 Da
• Isoelectric Point: 5.4773

>AT45278 WP_025205055.1 NZ_CP006580:c4269481-4269125 [Enterobacter ludwigii]
MNNHSESGTKPENPACLVWGLKCTITPCFGARLVQEGCRVHFLADRAGFNGTFSDDDALRLDQVFPLILKQLELMLTSGE
LFPQHEHCVTLYHNGLTCEADTLGSCGYIYIAIYPTQR

Nucleotide

• Length: 357 bp

>AT45278 NZ_CP006580:c4269481-4269125 [Enterobacter ludwigii]
ATGAACAATCACTCCGAATCCGGTACTAAGCCGGAGAACCCCGCCTGCCTGGTGTGGGGACTAAAGTGTACGATCACACC
CTGTTTCGGCGCTCGTCTGGTGCAGGAAGGCTGCCGGGTGCATTTTCTCGCTGACCGGGCAGGATTCAACGGTACTTTCA
GCGACGATGATGCGCTACGCCTGGATCAAGTCTTTCCGCTGATACTCAAGCAACTGGAGTTGATGCTCACCAGCGGAGAA
CTCTTTCCTCAGCATGAGCATTGCGTCACGCTATATCACAATGGGCTCACGTGTGAAGCCGATACTCTTGGTAGTTGTGG
CTACATATACATCGCCATTTATCCCACTCAACGCTAA