Detailed information of TA system TA045266

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 994574..995332
• Replicon: chromosome
• Accession: NZ_CP006580
• Organism: Enterobacter ludwigii strain P101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: M942_RS05020
• Protein ID: WP_025203168.1
• Coordinates: 994574..994966 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: M942_RS05025
• Protein ID: WP_131631904.1
• Coordinates: 995006..995332 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M942_RS05000	992205..992732	+	528	WP_044857798.1	LuxR family transcriptional regulator	-
M942_RS05010	993028..993876	-	849	WP_025203166.1	DUF4942 domain-containing protein	-
M942_RS05015	994086..994577	-	492	WP_025203167.1	hypothetical protein	-
M942_RS05020	994574..994966	-	393	WP_025203168.1	TA system toxin CbtA family protein	Toxin
M942_RS05025	995006..995332	-	327	WP_131631904.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
M942_RS05030	995389..995610	-	222	WP_025203170.1	DUF987 domain-containing protein	-
M942_RS05035	995607..996086	-	480	WP_025203171.1	DNA repair protein RadC	-
M942_RS05040	996097..996582	-	486	WP_025203172.1	antirestriction protein	-
M942_RS05045	996599..996853	-	255	WP_025203173.1	hypothetical protein	-
M942_RS05050	996853..997671	-	819	WP_025203174.1	DUF945 domain-containing protein	-
M942_RS05055	997763..997996	-	234	WP_025203175.1	DUF905 domain-containing protein	-
M942_RS05060	998054..998278	-	225	WP_025203176.1	hypothetical protein	-
M942_RS05065	998361..998873	-	513	WP_025203177.1	DUF4234 domain-containing protein	-
M942_RS05070	998932..999402	-	471	WP_025203178.1	hypothetical protein	-
M942_RS05075	999449..999760	-	312	WP_025203179.1	hypothetical protein	-
M942_RS05080	999796..1000056	-	261	WP_042866796.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-115)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14252.25 Da
• Isoelectric Point: 6.4538

>T45266 WP_025203168.1 NZ_CP006580:c994966-994574 [Enterobacter ludwigii]
MRILPVSAKRAAEICPSPGVSPVAVWLTLLTYLLDQHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTD
RRGFSAETQSPLISSIDILRARRATGLLKRSGYRAITDITTGQYSLESGQ

Nucleotide

• Length: 393 bp

>T45266 NZ_CP006580:c994966-994574 [Enterobacter ludwigii]
ATGAGAATTTTACCTGTATCCGCAAAACGGGCGGCTGAGATTTGCCCGTCACCTGGTGTGTCACCAGTGGCTGTGTGGCT
GACCCTACTGACGTACCTGCTGGACCAGCATTACGGTCTAACGCTGAACGACACACCGTTTGGCAATGACGGCGTGATCC
AGGAACACATTGACGCCGGTATTTCACTGTGTGACGCGGTGAATTTTATCGTGGAGAAATATGACCTGGTGCGTACTGAC
CGTCGTGGCTTCAGCGCAGAAACACAATCGCCACTTATCAGCAGTATCGATATACTTCGCGCCCGTCGTGCCACCGGGCT
GCTGAAACGTAGCGGTTACAGAGCCATCACCGATATCACCACCGGGCAATATTCACTGGAGTCCGGGCAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12147.00 Da
• Isoelectric Point: 7.6411

>AT45266 WP_131631904.1 NZ_CP006580:c995332-995006 [Enterobacter ludwigii]
IAEPWWGLRRNNTPCFGARLVQEGNWLHYLADRTSITGIFSDADLRRIDQVFPLLLKQLELKLISGELTPRRQHCVTLHA
KGLTCEADSLCSCGYVYIAIYPTSAPTA

Nucleotide

• Length: 327 bp

>AT45266 NZ_CP006580:c995332-995006 [Enterobacter ludwigii]
ATCGCTGAGCCCTGGTGGGGTCTGAGACGCAACAACACGCCCTGTTTTGGCGCGAGACTGGTACAGGAGGGCAACTGGCT
GCATTACCTGGCTGACCGCACCAGCATCACCGGCATCTTCAGTGACGCAGATTTACGCCGTATCGATCAGGTGTTTCCTC
TCTTGCTTAAGCAGCTTGAACTGAAACTGATCAGCGGTGAACTGACCCCACGCCGCCAGCATTGTGTCACGCTCCACGCG
AAAGGGCTGACCTGTGAAGCGGACAGTCTCTGCTCCTGTGGTTATGTCTATATCGCGATTTACCCGACATCTGCACCTAC
CGCATAA