Detailed information of TA system TA045262

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 66395..67150
• Replicon: chromosome
• Accession: NZ_CP006580
• Organism: Enterobacter ludwigii strain P101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: M942_RS00380
• Protein ID: WP_025202709.1
• Coordinates: 66773..67150 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: M942_RS00375
• Protein ID: WP_204247299.1
• Coordinates: 66395..66721 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M942_RS00340	63438..63902	+	465	WP_025202701.1	hypothetical protein	-
M942_RS00345	63989..64351	+	363	WP_025202702.1	hypothetical protein	-
M942_RS00350	64429..64650	+	222	WP_025202703.1	hypothetical protein	-
M942_RS00355	64706..64939	+	234	WP_025202704.1	DUF905 domain-containing protein	-
M942_RS00360	65145..65615	+	471	WP_025202705.1	antirestriction protein	-
M942_RS00365	65627..66106	+	480	WP_025202706.1	DNA repair protein RadC	-
M942_RS00370	66118..66339	+	222	WP_025202707.1	DUF987 domain-containing protein	-
M942_RS00375	66395..66721	+	327	WP_204247299.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
M942_RS00380	66773..67150	+	378	WP_025202709.1	TA system toxin CbtA family protein	Toxin
M942_RS00385	67147..67638	+	492	WP_025202710.1	hypothetical protein	-
M942_RS00390	67667..67879	+	213	WP_025202711.1	DUF957 domain-containing protein	-
M942_RS00395	67999..68970	-	972	WP_025202712.1	glycosyltransferase	-
M942_RS28025	69046..69210	-	165	WP_204247284.1	hypothetical protein	-
M942_RS00400	69500..70333	+	834	WP_025202713.1	DUF4942 domain-containing protein	-
M942_RS00405	70445..70681	+	237	WP_025202714.1	type II toxin-antitoxin system CcdA family antitoxin	-
M942_RS00410	70684..70998	+	315	WP_025202715.1	CcdB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14220.04 Da
• Isoelectric Point: 6.7261

>T45262 WP_025202709.1 NZ_CP006580:66773-67150 [Enterobacter ludwigii]
MQTQPLSSTREASPRPSPVEIWQQLLSHLLDRHYGLMLNDTPFGNDGVIQEHIDAGISLYDAVNFIVEKYDLVRTDRRGF
SAETQSPLLTSIDILRARKATGLMTRNDYRTVTNITTGKYREVQP

Nucleotide

• Length: 378 bp

>T45262 NZ_CP006580:66773-67150 [Enterobacter ludwigii]
ATGCAAACCCAACCACTATCCTCAACGCGGGAGGCTTCGCCGCGACCGTCCCCCGTGGAGATCTGGCAGCAACTTCTGAG
CCACCTGCTGGATCGTCACTACGGTCTGATGCTCAACGACACGCCATTTGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCTGGGATCTCACTATACGACGCGGTGAATTTCATCGTGGAGAAATACGATCTGGTGCGCACCGATCGCCGTGGTTTT
AGTGCAGAAACACAGTCTCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGCAA
CGATTACAGAACGGTGACCAACATCACCACCGGTAAATACCGCGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12083.84 Da
• Isoelectric Point: 6.6283

>AT45262 WP_204247299.1 NZ_CP006580:66395-66721 [Enterobacter ludwigii]
IAEPWWGLKRDFTPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPVLLKQLELMLVSGELNPRHQHCVTLYA
KGLICEADTLGSHGYVYLAIYPTPATTA

Nucleotide

• Length: 327 bp

>AT45262 NZ_CP006580:66395-66721 [Enterobacter ludwigii]
ATCGCTGAGCCATGGTGGGGACTTAAACGCGATTTTACCCCGTGTTTTGGCGCACGCCTGGTGCAGGAGGGCAACCGGTT
GCACTACCTGGCCGACCGCGCCAGTATCACCGGGCAGTTCAGCGACGCGGATTTACGCCATCTCGATCAGGCGTTTCCGG
TGTTGCTCAAACAACTGGAGCTGATGCTCGTTTCCGGTGAACTCAATCCCCGCCATCAGCACTGCGTCACGCTTTACGCA
AAAGGACTGATCTGTGAGGCCGACACCCTCGGCTCGCACGGGTACGTGTACCTGGCAATTTATCCAACACCAGCCACAAC
CGCGTAA