Detailed information of TA system TA045259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 261721..262364
• Replicon: plasmid pHS1
• Accession: NZ_CP006570
• Organism: Sodalis praecaptivus strain HS1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: W0I4B8
• Locus tag: SANT_RS22055
• Protein ID: WP_025424393.1
• Coordinates: 261721..262137 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: W0HZS3
• Locus tag: SANT_RS22060
• Protein ID: WP_025424394.1
• Coordinates: 262134..262364 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SANT_RS22045	258871..260667	-	1797	WP_025424391.1	malto-oligosyltrehalose trehalohydrolase	-
SANT_RS22050	261305..261652	+	348	WP_025424392.1	hypothetical protein	-
SANT_RS22055	261721..262137	-	417	WP_025424393.1	PIN domain-containing protein	Toxin
SANT_RS22060	262134..262364	-	231	WP_025424394.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SANT_RS22065	263945..264760	+	816	WP_025424395.1	fimbrial protein	-
SANT_RS22070	265581..265820	+	240	WP_025424396.1	hypothetical protein	-
SANT_RS22075	266193..266684	+	492	WP_025424397.1	carboxymuconolactone decarboxylase family protein	-
SANT_RS22080	266808..267191	+	384	WP_025424398.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..449897	449897

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14965.42 Da
• Isoelectric Point: 8.5441

>T45259 WP_025424393.1 NZ_CP006570:c262137-261721 [Sodalis praecaptivus]
MTKTFMLDTNICSFIMRELPVGVITRLEQAVLRRQRVVVSAITYAEMRFGAVGKKASPRHALLVESFCQRLDAVLAWDRA
AVDATTNIKITLAATGTPIGNNDAAIAGHAIAAGAVLVTNNTREFERVPGLIIEDWGL

Nucleotide

• Length: 417 bp

>T45259 NZ_CP006570:c262137-261721 [Sodalis praecaptivus]
TTGACTAAGACGTTTATGTTGGACACCAATATTTGCTCCTTTATCATGCGCGAGCTGCCGGTCGGGGTGATCACTCGTCT
GGAACAAGCGGTACTCCGCCGTCAGCGAGTTGTGGTATCGGCCATCACCTATGCCGAGATGCGCTTTGGCGCCGTGGGTA
AAAAAGCCTCGCCGCGTCATGCGCTGCTGGTTGAGTCGTTCTGCCAGCGGCTGGATGCGGTACTGGCTTGGGACCGGGCC
GCGGTGGATGCCACTACCAACATTAAAATCACGCTGGCGGCGACCGGTACGCCGATCGGAAATAACGACGCGGCAATTGC
CGGCCACGCGATTGCGGCCGGTGCCGTGCTAGTGACAAATAATACCAGGGAGTTTGAGCGGGTACCGGGGCTGATTATTG
AGGATTGGGGTCTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8753.96 Da
• Isoelectric Point: 4.5742

>AT45259 WP_025424394.1 NZ_CP006570:c262364-262134 [Sodalis praecaptivus]
MRIVSVFKNGNNRAIRLPRDLDFAGVNELEILRDGDMLILRPVKPTWGSFLQQEKADATFLAEREDVISDEGRFDF

Nucleotide

• Length: 231 bp

>AT45259 NZ_CP006570:c262364-262134 [Sodalis praecaptivus]
ATGAGAATTGTTTCCGTGTTTAAAAATGGCAATAACCGGGCTATTCGGCTTCCCCGAGATTTGGATTTTGCAGGCGTCAA
TGAGCTGGAGATCCTCCGCGACGGCGATATGCTTATCCTTCGGCCAGTAAAACCCACCTGGGGATCGTTCTTGCAGCAGG
AGAAAGCTGACGCAACCTTTTTGGCCGAACGAGAGGACGTGATAAGCGATGAGGGGAGATTTGATTTTTGA

Structures

Toxin

• AlphaFold DB: W0I4B8

Antitoxin

• AlphaFold DB: W0HZS3