Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3460979..3461562 Replicon chromosome
Accession NZ_CP006262
Organism Escherichia coli O145:H28 str. RM13516

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U9LIA0
Locus tag ECRM13516_RS17700 Protein ID WP_000254747.1
Coordinates 3460979..3461314 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECRM13516_RS17705 Protein ID WP_000581937.1
Coordinates 3461314..3461562 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM13516_RS17685 3456866..3458164 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECRM13516_RS17690 3458252..3459889 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECRM13516_RS17695 3460117..3460908 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
ECRM13516_RS17700 3460979..3461314 - 336 WP_000254747.1 endoribonuclease MazF Toxin
ECRM13516_RS17705 3461314..3461562 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECRM13516_RS17710 3461640..3463874 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECRM13516_RS17715 3463922..3465223 - 1302 WP_000046821.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T45222 WP_000254747.1 NZ_CP006262:c3461314-3460979 [Escherichia coli O145:H28 str. RM13516]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T45222 NZ_CP006262:c3461314-3460979 [Escherichia coli O145:H28 str. RM13516]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTATGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT45222 WP_000581937.1 NZ_CP006262:c3461562-3461314 [Escherichia coli O145:H28 str. RM13516]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT45222 NZ_CP006262:c3461562-3461314 [Escherichia coli O145:H28 str. RM13516]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LIA0

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References