Detailed information of TA system TA045200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 9336..9922
• Replicon: plasmid unnamed
• Accession: NZ_CP006257
• Organism: Pseudomonas syringae pv. syringae HS191

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A3M2XXJ5
• Locus tag: PSYRH_RS25860
• Protein ID: WP_046720389.1
• Coordinates: 9336..9635 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PSYRH_RS25865
• Protein ID: WP_046720390.1
• Coordinates: 9632..9922 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSYRH_RS25835	5188..6267	+	1080	WP_046720385.1	restriction endonuclease	-
PSYRH_RS25840	6279..6605	+	327	WP_046720386.1	FxLYD domain-containing protein	-
PSYRH_RS25845	6639..6944	+	306	WP_052755373.1	hypothetical protein	-
PSYRH_RS25850	6988..7191	+	204	WP_046720387.1	carbon storage regulator	-
PSYRH_RS25855	7531..9156	-	1626	WP_046720388.1	methyl-accepting chemotaxis protein	-
PSYRH_RS25860	9336..9635	+	300	WP_046720389.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSYRH_RS25865	9632..9922	+	291	WP_046720390.1	putative addiction module antidote protein	Antitoxin
PSYRH_RS27655	10403..10690	+	288	WP_080348823.1	hypothetical protein	-
PSYRH_RS25870	10783..11193	+	411	WP_016569388.1	hypothetical protein	-
PSYRH_RS25875	11531..11731	+	201	WP_046720391.1	hypothetical protein	-
PSYRH_RS25880	11844..12494	+	651	WP_017279394.1	AAA family ATPase	-
PSYRH_RS25885	12484..12771	+	288	WP_025391104.1	hypothetical protein	-
PSYRH_RS25890	13009..13650	+	642	WP_046720392.1	SOS response-associated peptidase	-
PSYRH_RS25895	13837..14499	+	663	WP_046720393.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..52548	52548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11164.79 Da
• Isoelectric Point: 10.3737

>T45200 WP_046720389.1 NZ_CP006257:9336-9635 [Pseudomonas syringae pv. syringae HS191]
MIEIKQTATFMNRESKLKDQRAKAAIAARIFRLANGLPGDVSPVGQEVSELRIHHGPGYRVYFQQRGTEIVILLCGGDKS
SQTRDIETAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T45200 NZ_CP006257:9336-9635 [Pseudomonas syringae pv. syringae HS191]
ATGATCGAAATCAAACAAACCGCCACTTTCATGAACCGGGAGAGCAAGCTGAAGGATCAGCGGGCAAAGGCCGCAATTGC
CGCTCGCATCTTCCGTTTGGCGAATGGTCTACCCGGCGACGTGTCACCAGTCGGCCAAGAAGTGAGCGAGTTGAGGATTC
ACCATGGCCCAGGCTATCGGGTTTACTTCCAGCAGCGCGGGACAGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGACCAGGGACATAGAAACGGCGAAACGCCTAGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10303.83 Da
• Isoelectric Point: 4.7527

>AT45200 WP_046720390.1 NZ_CP006257:9632-9922 [Pseudomonas syringae pv. syringae HS191]
MTEAIYDYDPAQALDGPEAIAVFIADALDTGDAAYIAKAMGVVARTKGMTELAKETGLAREQLYKSFSERGNPTLKTMLV
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT45200 NZ_CP006257:9632-9922 [Pseudomonas syringae pv. syringae HS191]
ATGACTGAGGCAATTTATGACTACGATCCCGCGCAAGCGCTGGACGGCCCAGAGGCGATAGCGGTGTTCATCGCGGACGC
CTTGGACACTGGAGACGCCGCCTATATCGCTAAGGCTATGGGAGTGGTTGCCCGCACCAAGGGAATGACCGAGTTGGCCA
AGGAAACGGGGCTGGCTAGAGAGCAGCTCTACAAGTCGTTCAGCGAACGCGGGAACCCTACACTGAAAACCATGCTGGTA
GTGATGCGGGCGCTGGGAGTTGATTTGACCGCCCGGCCGCATGCGCAATAA

Structures

Toxin

• AlphaFold DB: A0A3M2XXJ5