Detailed information of TA system TA045195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2709408..2710045
• Replicon: chromosome
• Accession: NZ_CP006058
• Organism: Bacillus amyloliquefaciens UMAF6639

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAMY6639_RS13130
• Protein ID: WP_003156187.1
• Coordinates: 2709695..2710045 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAMY6639_RS13125
• Protein ID: WP_003156188.1
• Coordinates: 2709408..2709689 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMY6639_RS13105	2705773..2706372	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BAMY6639_RS13110	2706465..2706830	+	366	WP_053104569.1	holo-ACP synthase	-
BAMY6639_RS13115	2706995..2708002	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BAMY6639_RS13120	2708119..2709288	+	1170	WP_061861177.1	alanine racemase	-
BAMY6639_RS13125	2709408..2709689	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAMY6639_RS13130	2709695..2710045	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAMY6639_RS13135	2710163..2710984	+	822	WP_015416874.1	STAS domain-containing protein	-
BAMY6639_RS13140	2710989..2711354	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAMY6639_RS13145	2711357..2711758	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAMY6639_RS13150	2711770..2712777	+	1008	WP_015416875.1	PP2C family protein-serine/threonine phosphatase	-
BAMY6639_RS13155	2712841..2713170	+	330	WP_033575044.1	anti-sigma factor antagonist	-
BAMY6639_RS13160	2713167..2713649	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BAMY6639_RS13165	2713615..2714403	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BAMY6639_RS13170	2714403..2715005	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T45195 WP_003156187.1 NZ_CP006058:2709695-2710045 [Bacillus amyloliquefaciens UMAF6639]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T45195 NZ_CP006058:2709695-2710045 [Bacillus amyloliquefaciens UMAF6639]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT45195 WP_003156188.1 NZ_CP006058:2709408-2709689 [Bacillus amyloliquefaciens UMAF6639]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT45195 NZ_CP006058:2709408-2709689 [Bacillus amyloliquefaciens UMAF6639]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5