Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 502209..502845 Replicon chromosome
Accession NZ_CP005997
Organism Bacillus subtilis TO-A

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M036_RS02540 Protein ID WP_003156187.1
Coordinates 502495..502845 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag M036_RS02535 Protein ID WP_003225183.1
Coordinates 502209..502490 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M036_RS02515 498568..499167 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
M036_RS02520 499262..499627 + 366 WP_003234281.1 holo-ACP synthase -
M036_RS02525 499793..500809 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
M036_RS02530 500924..502093 + 1170 WP_003234284.1 alanine racemase -
M036_RS02535 502209..502490 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M036_RS02540 502495..502845 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M036_RS02545 502960..503784 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
M036_RS02550 503789..504154 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
M036_RS02555 504158..504559 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
M036_RS02560 504571..505578 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
M036_RS02565 505640..505969 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
M036_RS02570 505966..506448 + 483 WP_003234299.1 anti-sigma B factor RsbW -
M036_RS02575 506414..507202 + 789 WP_048217624.1 RNA polymerase sigma factor SigB -
M036_RS02580 507202..507801 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T45154 WP_003156187.1 NZ_CP005997:502495-502845 [Bacillus subtilis TO-A]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T45154 NZ_CP005997:502495-502845 [Bacillus subtilis TO-A]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT45154 WP_003225183.1 NZ_CP005997:502209-502490 [Bacillus subtilis TO-A]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT45154 NZ_CP005997:502209-502490 [Bacillus subtilis TO-A]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References