Detailed information of TA system TA045152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 3337..3817
• Replicon: plasmid pACA1.1
• Accession: NZ_CP005988
• Organism: Acidithiobacillus caldus ATCC 51756

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: ACAty_RS14570
• Protein ID: WP_040131488.1
• Coordinates: 3337..3609 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A060A3P0
• Locus tag: ACAty_RS14575
• Protein ID: WP_040131490.1
• Coordinates: 3593..3817 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACAty_RS14555	313..1626	+	1314	WP_004872540.1	replication protein	-
ACAty_RS14560	2612..2947	+	336	WP_040131484.1	DNA-binding protein	-
ACAty_RS14565	3027..3251	+	225	WP_040131486.1	hypothetical protein	-
ACAty_RS14570	3337..3609	-	273	WP_040131488.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACAty_RS14575	3593..3817	-	225	WP_040131490.1	TraY domain-containing protein	Antitoxin
ACAty_RS15655	4486..4718	-	233	Protein_5	superinfection immunity protein	-
ACAty_RS14585	5040..5525	-	486	WP_040131494.1	hypothetical protein	-
ACAty_RS14590	5635..5949	+	315	WP_049784793.1	hypothetical protein	-
ACAty_RS15660	6140..6480	-	341	Protein_8	integration host factor subunit alpha	-
ACAty_RS14600	6693..7028	-	336	WP_040131497.1	conjugal transfer protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..27536	27536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10232.00 Da
• Isoelectric Point: 10.5146

>T45152 WP_040131488.1 NZ_CP005988:c3609-3337 [Acidithiobacillus caldus ATCC 51756]
MVWQIEIGDKAKKDLAALDRAVAKRITAFLRERVAQLDDPRSIGEALKGSKLGAFWKYRVGDYRIIASIEDGALCILVVR
IGNRREVYRK

Nucleotide

• Length: 273 bp

>T45152 NZ_CP005988:c3609-3337 [Acidithiobacillus caldus ATCC 51756]
TTGGTCTGGCAGATTGAGATCGGCGATAAGGCCAAGAAAGACTTGGCTGCGCTGGACAGAGCCGTCGCCAAGCGCATCAC
GGCTTTTCTGCGAGAGCGTGTCGCGCAACTCGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGCTCCAAGCTGGGAG
CCTTCTGGAAGTACCGTGTCGGCGATTACCGCATCATTGCCAGCATTGAGGACGGCGCGTTGTGCATCCTCGTTGTGCGC
ATCGGCAATCGCAGGGAGGTATACCGCAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8459.58 Da
• Isoelectric Point: 4.5098

>AT45152 WP_040131490.1 NZ_CP005988:c3817-3593 [Acidithiobacillus caldus ATCC 51756]
VLAIRLPAEVEARLEALARATGRTKTFYAREAILEHLDDLEDLYLAEQRLMDIRAGRSRTYTLDEVERDLGLAD

Nucleotide

• Length: 225 bp

>AT45152 NZ_CP005988:c3817-3593 [Acidithiobacillus caldus ATCC 51756]
GTGCTTGCCATTCGACTGCCCGCTGAAGTGGAGGCCCGCCTGGAAGCACTGGCTCGGGCCACCGGGCGCACCAAAACTTT
CTATGCCCGCGAAGCCATCCTTGAGCACTTGGACGACCTCGAAGATTTGTACCTTGCGGAGCAACGCCTGATGGACATTC
GCGCAGGCCGCAGCCGCACCTACACGCTGGACGAAGTAGAGCGCGATCTTGGTCTGGCAGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A060A3P0