Detailed information of TA system TA045150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 1908773..1909443
• Replicon: chromosome
• Accession: NZ_CP005986
• Organism: Acidithiobacillus caldus ATCC 51756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ACAty_RS09335
• Protein ID: WP_198408160.1
• Coordinates: 1909009..1909443 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A060A0T8
• Locus tag: ACAty_RS09330
• Protein ID: WP_038472114.1
• Coordinates: 1908773..1909015 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACAty_RS15910	1904570..1905340	+	771	WP_153801825.1	hypothetical protein	-
ACAty_RS09305	1905313..1905810	-	498	WP_038472112.1	hypothetical protein	-
ACAty_RS09310	1905807..1906697	-	891	WP_004872983.1	TcpQ domain-containing protein	-
ACAty_RS09315	1906757..1907647	-	891	WP_153801854.1	P-type conjugative transfer ATPase TrbB	-
ACAty_RS09320	1907761..1908285	-	525	WP_153801855.1	hypothetical protein	-
ACAty_RS09325	1908341..1908709	-	369	WP_051620786.1	hypothetical protein	-
ACAty_RS09330	1908773..1909015	+	243	WP_038472114.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ACAty_RS09335	1909009..1909443	+	435	WP_198408160.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACAty_RS09340	1909440..1909871	+	432	WP_004872993.1	hypothetical protein	-
ACAty_RS09345	1909964..1910833	+	870	Protein_1857	type I restriction enzyme HsdR N-terminal domain-containing protein	-
ACAty_RS15915	1910922..1911449	+	528	WP_004872996.1	hypothetical protein	-
ACAty_RS15920	1911459..1912073	+	615	WP_051620788.1	transposase	-
ACAty_RS09355	1912288..1912503	-	216	WP_153801827.1	hypothetical protein	-
ACAty_RS15455	1912657..1913364	-	708	WP_153801856.1	PilZ domain-containing protein	-
ACAty_RS15925	1913921..1914304	-	384	WP_038472118.1	CZB domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 16333.73 Da
• Isoelectric Point: 5.1965

>T45150 WP_198408160.1 NZ_CP005986:1909009-1909443 [Acidithiobacillus caldus ATCC 51756]
VVTVGGYLLDTNIVSELRRKRPEPKLLQWFEAVDDDQLYLSVLTTGEIRRGISRVKDPEKQKELSDWLEITLLPWLGSHL
LSVDRHVADVWGNLCGIADRPLPTIDSLLAATAMAHHLALVTRNTSDFQFPELPIINPWDSNLR

Nucleotide

• Length: 435 bp

>T45150 NZ_CP005986:1909009-1909443 [Acidithiobacillus caldus ATCC 51756]
GTGGTGACCGTCGGCGGCTATTTGCTGGACACCAATATCGTGTCCGAATTACGCCGTAAACGGCCTGAGCCGAAATTGCT
GCAATGGTTCGAAGCGGTCGATGACGATCAGCTGTACCTCAGCGTGCTGACGACAGGGGAAATCCGTCGGGGCATCTCCA
GGGTAAAAGACCCAGAAAAACAGAAAGAACTCTCTGATTGGCTGGAAATCACCCTGCTTCCCTGGCTGGGTTCCCATCTT
CTGTCCGTGGATCGGCATGTCGCCGACGTTTGGGGCAACCTGTGTGGAATCGCCGATCGTCCATTACCAACGATTGACAG
CCTGTTGGCGGCAACCGCCATGGCCCATCATCTCGCACTGGTCACCCGGAACACATCCGATTTTCAATTCCCCGAATTAC
CGATTATCAACCCCTGGGACAGCAATTTACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9262.54 Da
• Isoelectric Point: 10.1954

>AT45150 WP_038472114.1 NZ_CP005986:1908773-1909015 [Acidithiobacillus caldus ATCC 51756]
MPVWALQDAKAKFSQLVRRAQSEGPQTISVRGEPAVVMLSVETYRRLQPNRRSFVEIVRSGPDTQLDLHREQTTVRDIEW

Nucleotide

• Length: 243 bp

>AT45150 NZ_CP005986:1908773-1909015 [Acidithiobacillus caldus ATCC 51756]
ATGCCAGTTTGGGCATTGCAAGACGCCAAAGCAAAATTCAGCCAGCTAGTTCGCCGCGCCCAGTCGGAGGGTCCGCAAAC
CATCAGCGTGCGTGGCGAACCGGCAGTGGTGATGCTCTCCGTAGAAACCTACCGTCGGCTCCAACCGAACCGGCGGAGCT
TCGTGGAGATCGTCCGTAGCGGTCCAGATACCCAGCTGGATCTGCACCGCGAACAGACAACCGTTCGGGATATCGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A060A0T8