Detailed information of TA system TA045149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1881497..1882089
• Replicon: chromosome
• Accession: NZ_CP005986
• Organism: Acidithiobacillus caldus ATCC 51756

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F9ZMS0
• Locus tag: ACAty_RS09200
• Protein ID: WP_014002702.1
• Coordinates: 1881895..1882089 (-)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: F9ZMS1
• Locus tag: ACAty_RS09195
• Protein ID: WP_004872948.1
• Coordinates: 1881497..1881898 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACAty_RS09170	1876573..1877190	-	618	WP_082179267.1	hypothetical protein	-
ACAty_RS09175	1877166..1877510	-	345	WP_004872943.1	hypothetical protein	-
ACAty_RS09180	1877443..1878336	-	894	WP_038472095.1	hypothetical protein	-
ACAty_RS09185	1878532..1879365	-	834	WP_004872944.1	hypothetical protein	-
ACAty_RS09190	1879365..1880777	-	1413	WP_038472074.1	RRXRR domain-containing protein	-
ACAty_RS09195	1881497..1881898	-	402	WP_004872948.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ACAty_RS09200	1881895..1882089	-	195	WP_014002702.1	type II toxin-antitoxin system HicA family toxin	Toxin
ACAty_RS09205	1882332..1884809	-	2478	WP_004872954.1	conjugal transfer protein TrbE	-
ACAty_RS09210	1884812..1885183	-	372	WP_004872955.1	VirB3 family type IV secretion system protein	-
ACAty_RS09215	1885202..1885594	-	393	WP_004872956.1	hypothetical protein	-
ACAty_RS09220	1885930..1886190	+	261	WP_038472100.1	type II toxin-antitoxin system HicA family toxin	-
ACAty_RS09225	1886187..1886507	+	321	Protein_1831	type II toxin-antitoxin system HicB family antitoxin	-
ACAty_RS09230	1886638..1886913	+	276	WP_051620776.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1867550..1888029	20479

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7000.19 Da
• Isoelectric Point: 10.8255

>T45149 WP_014002702.1 NZ_CP005986:c1882089-1881895 [Acidithiobacillus caldus ATCC 51756]
MNSGDLIKQLLADGWVVRATKGSHHIYIHPNKPGHITVPHPKKDLGVGLVNKILKQAGLKRSES

Nucleotide

• Length: 195 bp

>T45149 NZ_CP005986:c1882089-1881895 [Acidithiobacillus caldus ATCC 51756]
ATGAACAGCGGTGATCTGATCAAGCAGTTGCTGGCCGATGGATGGGTGGTGCGAGCCACGAAAGGCTCGCACCACATTTA
TATTCATCCGAACAAGCCAGGGCATATCACTGTGCCGCATCCCAAGAAGGATCTCGGGGTGGGCTTGGTCAACAAGATCC
TGAAACAGGCTGGCCTGAAACGGAGCGAATCATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14247.10 Da
• Isoelectric Point: 4.4575

>AT45149 WP_004872948.1 NZ_CP005986:c1881898-1881497 [Acidithiobacillus caldus ATCC 51756]
MNYPIAIEPGDDHHAYGVVVPDLPGCFSAGDTLDEAIANAHEAIALWLETVMDDGGTIPAPSSVESLQKQPEFSGWIWAV
VPVDLAKLSDRVERVNITLPARVLRRIDAAARAAGESRSGYLARCAIMGEPHS

Nucleotide

• Length: 402 bp

>AT45149 NZ_CP005986:c1881898-1881497 [Acidithiobacillus caldus ATCC 51756]
ATGAATTATCCCATTGCCATCGAACCTGGGGATGACCACCACGCTTATGGCGTGGTCGTCCCCGACCTGCCGGGGTGCTT
CTCGGCGGGAGATACGTTGGACGAAGCGATTGCCAACGCCCATGAGGCTATTGCGTTGTGGCTGGAAACCGTGATGGATG
ACGGCGGTACGATTCCTGCGCCATCCTCCGTGGAGTCGCTCCAGAAGCAGCCGGAGTTTTCCGGATGGATCTGGGCTGTG
GTACCGGTGGATCTGGCGAAGTTGTCCGATCGGGTGGAGCGCGTCAACATCACGCTGCCCGCCCGGGTCCTGCGCCGCAT
CGATGCGGCGGCCCGGGCTGCCGGAGAATCCCGCTCAGGCTATCTGGCGCGCTGTGCAATAATGGGCGAACCACATTCGT
GA

Structures

Toxin

• AlphaFold DB: A0A1E7YRY8

Antitoxin

• AlphaFold DB: A0A1E7YRV7