Detailed information of TA system TA045148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1327378..1327973
• Replicon: chromosome
• Accession: NZ_CP005986
• Organism: Acidithiobacillus caldus ATCC 51756

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F9ZNF3
• Locus tag: ACAty_RS06495
• Protein ID: WP_014002921.1
• Coordinates: 1327378..1327674 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ACAty_RS06500
• Protein ID: WP_004872065.1
• Coordinates: 1327680..1327973 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACAty_RS06475	1322833..1323750	+	918	WP_004872060.1	magnesium transporter	-
ACAty_RS06480	1323772..1324965	-	1194	WP_004872061.1	divalent metal cation transporter	-
ACAty_RS06485	1325225..1325932	-	708	WP_004872063.1	MgtC/SapB family protein	-
ACAty_RS15230	1325996..1326205	-	210	WP_103415044.1	hypothetical protein	-
ACAty_RS06495	1327378..1327674	+	297	WP_014002921.1	addiction module antitoxin RelB	Toxin
ACAty_RS06500	1327680..1327973	+	294	WP_004872065.1	putative addiction module antidote protein	Antitoxin
ACAty_RS06505	1328180..1329124	+	945	WP_169737325.1	IS5 family transposase	-
ACAty_RS06510	1329362..1330369	-	1008	WP_153801806.1	GTP-binding protein	-
ACAty_RS06515	1330645..1332270	-	1626	WP_004872068.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11235.09 Da
• Isoelectric Point: 9.9555

>T45148 WP_014002921.1 NZ_CP005986:1327378-1327674 [Acidithiobacillus caldus ATCC 51756]
MKEIIKSDIFDRWLIALRDRRARARIEVRIRRLSLGNPGDVKPVGEGVLEIRIDYDPGYHVYFMSRGPLVIVLLCAGDKS
SQDRDIALAKNIAAQWKE

Nucleotide

• Length: 297 bp

>T45148 NZ_CP005986:1327378-1327674 [Acidithiobacillus caldus ATCC 51756]
ATGAAGGAAATCATCAAATCCGATATTTTCGATCGCTGGCTTATCGCACTTCGTGACCGACGAGCCCGAGCAAGGATCGA
GGTGCGAATTCGGCGATTGAGCCTTGGTAATCCCGGAGATGTAAAACCAGTAGGGGAAGGCGTATTGGAAATACGGATTG
ATTACGATCCAGGTTACCACGTCTATTTTATGAGTCGTGGCCCATTGGTCATAGTACTGCTTTGTGCTGGCGACAAAAGC
AGTCAGGACCGGGATATTGCCTTGGCCAAGAACATTGCAGCACAGTGGAAGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10471.97 Da
• Isoelectric Point: 5.0634

>AT45148 WP_004872065.1 NZ_CP005986:1327680-1327973 [Acidithiobacillus caldus ATCC 51756]
MDIQFSRYDTVDYLQTEEDMVAYLDAVMEDGDPALIAAALGDIARARNLSRLAREVGMSRQGLDKALSGKGNPSLATVLK
VAKALGLRLAFQTARQG

Nucleotide

• Length: 294 bp

>AT45148 NZ_CP005986:1327680-1327973 [Acidithiobacillus caldus ATCC 51756]
ATGGACATCCAATTCAGTCGCTATGACACGGTAGACTACCTTCAGACCGAGGAAGACATGGTGGCTTATCTCGATGCGGT
GATGGAAGACGGCGACCCTGCATTGATTGCCGCAGCGCTGGGGGACATAGCCCGCGCCCGCAATCTCAGCCGCCTCGCCC
GAGAAGTCGGAATGAGCCGCCAGGGGCTGGACAAGGCCCTTTCGGGCAAAGGCAACCCCAGCTTGGCTACGGTCCTCAAG
GTCGCCAAGGCGCTCGGGCTAAGACTGGCCTTTCAAACAGCCAGACAAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A1E7YSJ9