Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 6069233..6069837 Replicon chromosome
Accession NZ_CP005975
Organism Pseudomonas simiae strain PICF7

Toxin (Protein)


Gene name hicA Uniprot ID A0A1N7UCS2
Locus tag PFLUOLIPICF7_RS27715 Protein ID WP_010211777.1
Coordinates 6069649..6069837 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1N7UII5
Locus tag PFLUOLIPICF7_RS27710 Protein ID WP_010211776.1
Coordinates 6069233..6069637 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFLUOLIPICF7_RS27690 6064258..6065262 + 1005 WP_010211770.1 sulfate ABC transporter substrate-binding protein -
PFLUOLIPICF7_RS27695 6065392..6066006 - 615 WP_021492218.1 hypothetical protein -
PFLUOLIPICF7_RS27700 6066183..6067379 + 1197 WP_021492219.1 MFS transporter -
PFLUOLIPICF7_RS27705 6067389..6068927 - 1539 WP_045792370.1 M10 family metallopeptidase C-terminal domain-containing protein -
PFLUOLIPICF7_RS27710 6069233..6069637 - 405 WP_010211776.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PFLUOLIPICF7_RS27715 6069649..6069837 - 189 WP_010211777.1 type II toxin-antitoxin system HicA family toxin Toxin
PFLUOLIPICF7_RS27720 6070157..6070633 + 477 WP_045792371.1 GNAT family N-acetyltransferase -
PFLUOLIPICF7_RS27725 6070658..6071230 - 573 WP_010211780.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
PFLUOLIPICF7_RS27730 6071510..6071836 - 327 WP_045792372.1 lipoprotein -
PFLUOLIPICF7_RS27735 6071853..6073040 - 1188 WP_045792373.1 c-type cytochrome biogenesis protein CcmI -
PFLUOLIPICF7_RS27740 6073033..6073506 - 474 WP_010211783.1 cytochrome c-type biogenesis protein CcmH -
PFLUOLIPICF7_RS27745 6073503..6074039 - 537 WP_010211784.1 DsbE family thiol:disulfide interchange protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7161.38 Da        Isoelectric Point: 11.0014

>T45146 WP_010211777.1 NZ_CP005975:c6069837-6069649 [Pseudomonas simiae]
VQSRLLIKELQEAGWVLDRVAGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRKRAGLFQF

Download         Length: 189 bp

>T45146 NZ_CP005975:c6069837-6069649 [Pseudomonas simiae]
GTGCAAAGCAGGTTATTGATCAAAGAACTGCAAGAGGCGGGTTGGGTGTTGGACCGGGTCGCGGGCAGTCATCATATTTT
TACTCACCGCTATAACCCGTACACCATTCCGGTACCCCACCCAAAAAAGGACCTGCCGCTGGGCACTGTCAAAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14683.64 Da        Isoelectric Point: 4.6000

>AT45146 WP_010211776.1 NZ_CP005975:c6069637-6069233 [Pseudomonas simiae]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPLPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT45146 NZ_CP005975:c6069637-6069233 [Pseudomonas simiae]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATTCAGATCCCCGACATCCCTGGCGCAGT
CACCGCAGGGGACAGCTTTGAGGAGGCCTATAACGCTGCGGTGGAAGTCGCCCACATCATGCTGCAAGAGATTGCCGCAG
AGGGTGGACCGATCCCCCTGCCAACGTCGGTGGCCAACCATCATGCGCACGAAGATTACGCCGGCATGGGCTGGGGGATG
TTGGAGTTGGACATCTCGCCCTACCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGTTATGTGATCCAGCGCAT
CGACCGTTATGTGCGTGAGCACAAGGTCAAGAGTCGCTCTTCCTTTCTGGCGGATGCCGCGCTGGAGAAGCTGGTGCGGT
CCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1N7UCS2

Antitoxin

Source ID Structure
AlphaFold DB A0A1N7UII5

References