45141

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 35922..36615 Replicon plasmid unnamed
Accession NZ_CP005961
Organism Pseudomonas mandelii JR-1

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag OU5_RS30980 Protein ID WP_003151133.1
Coordinates 36238..36615 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag OU5_RS30975 Protein ID WP_001172026.1
Coordinates 35922..36257 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU5_RS30945 32893..33288 - 396 WP_104455936.1 transposase -
OU5_RS30950 33455..34369 - 915 WP_001736514.1 HD domain-containing protein -
OU5_RS33805 34416..34727 - 312 WP_010465812.1 DUF3391 domain-containing protein -
OU5_RS30960 34845..35225 - 381 WP_001054412.1 hypothetical protein -
OU5_RS30965 35222..35548 - 327 WP_000091614.1 hypothetical protein -
OU5_RS30970 35572..35907 - 336 WP_000741275.1 hypothetical protein -
OU5_RS30975 35922..36257 - 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
OU5_RS30980 36238..36615 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OU5_RS30985 36807..37409 + 603 WP_010465829.1 recombinase family protein -
OU5_RS30990 37393..40422 + 3030 WP_010465834.1 Tn3 family transposase -
OU5_RS30995 40452..41258 - 807 Protein_44 TonB-dependent receptor plug domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..410512 410512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T45141 WP_003151133.1 NZ_CP005961:c36615-36238 [Pseudomonas mandelii JR-1]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T45141 NZ_CP005961:c36615-36238 [Pseudomonas mandelii JR-1]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT45141 WP_001172026.1 NZ_CP005961:c36257-35922 [Pseudomonas mandelii JR-1]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT45141 NZ_CP005961:c36257-35922 [Pseudomonas mandelii JR-1]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References