Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 6427195..6427799 Replicon chromosome
Accession NZ_CP005960
Organism Pseudomonas mandelii JR-1

Toxin (Protein)


Gene name PumA Uniprot ID A0A855KA05
Locus tag OU5_RS29085 Protein ID WP_010455649.1
Coordinates 6427506..6427799 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A024EJX4
Locus tag OU5_RS29080 Protein ID WP_010455651.1
Coordinates 6427195..6427503 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU5_RS35160 6422567..6422806 + 240 Protein_5764 electron transporter SenC -
OU5_RS29060 6422910..6424403 + 1494 WP_010455659.1 HAMP domain-containing histidine kinase -
OU5_RS29065 6424372..6425799 + 1428 WP_010455657.1 sigma-54 dependent transcriptional regulator -
OU5_RS29070 6425803..6426189 - 387 WP_010455655.1 hypothetical protein -
OU5_RS29075 6426369..6427181 + 813 WP_010455653.1 class I SAM-dependent methyltransferase -
OU5_RS29080 6427195..6427503 - 309 WP_010455651.1 putative addiction module antidote protein Antitoxin
OU5_RS29085 6427506..6427799 - 294 WP_010455649.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OU5_RS29090 6428898..6429590 - 693 WP_010455647.1 hypothetical protein -
OU5_RS29095 6429706..6430497 - 792 WP_042932752.1 ChbG/HpnK family deacetylase -
OU5_RS29100 6430469..6431686 - 1218 WP_010455642.1 GNAT family N-acetyltransferase -
OU5_RS29105 6431691..6432380 - 690 WP_010455641.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(5-96)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10646.39 Da        Isoelectric Point: 10.8628

>T45140 WP_010455649.1 NZ_CP005960:c6427799-6427506 [Pseudomonas mandelii JR-1]
MNYIVQQTMIFVAWHASVRDLRAKIAIARRIDRASAGNLGDIKPVGDGVSEMRVDVGAGYRVYFTLRNSVVIVLLAGGDK
SSQTADIRRAKKLAKEV

Download         Length: 294 bp

>T45140 NZ_CP005960:c6427799-6427506 [Pseudomonas mandelii JR-1]
ATGAATTATATTGTTCAACAAACTATGATCTTCGTGGCATGGCATGCCTCAGTTCGTGACTTGCGGGCGAAGATTGCTAT
CGCTCGTCGCATTGATCGTGCCTCTGCAGGTAACCTGGGTGATATCAAGCCGGTGGGAGACGGCGTTTCCGAAATGCGCG
TGGACGTCGGCGCCGGCTATCGGGTCTACTTCACTTTGCGAAACAGTGTCGTCATTGTGCTTTTGGCCGGTGGTGATAAA
TCTTCGCAAACCGCCGATATCAGGCGGGCTAAAAAATTGGCAAAGGAGGTTTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11043.61 Da        Isoelectric Point: 5.0482

>AT45140 WP_010455651.1 NZ_CP005960:c6427503-6427195 [Pseudomonas mandelii JR-1]
MSQTLTTFDMAALLDSDEAISEYLSQVLAEGDNEEFLRAIGYVVKARGMTQIAKDSGMGRESLYKAFAPGAKPRFDTVLK
VIHALGIDLCAQPGHSINHSVL

Download         Length: 309 bp

>AT45140 NZ_CP005960:c6427503-6427195 [Pseudomonas mandelii JR-1]
ATGAGCCAGACTTTGACTACTTTCGATATGGCGGCACTGCTGGACAGTGACGAAGCCATTAGCGAGTACCTCTCACAAGT
GCTCGCAGAGGGCGATAACGAGGAATTTCTTCGTGCTATCGGATACGTGGTGAAAGCGCGCGGAATGACACAGATTGCCA
AGGACTCGGGTATGGGTCGCGAAAGCCTGTACAAGGCCTTCGCGCCCGGTGCAAAACCACGCTTTGATACGGTTTTGAAG
GTCATCCATGCCCTCGGCATCGATCTCTGTGCGCAGCCAGGTCATTCGATCAATCACTCGGTGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855KA05

Antitoxin

Source ID Structure
AlphaFold DB A0A024EJX4

References