Detailed information of TA system TA045138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3570148..3570785
• Replicon: chromosome
• Accession: NZ_CP005960
• Organism: Pseudomonas mandelii JR-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: OU5_RS15790
• Protein ID: WP_010464625.1
• Coordinates: 3570378..3570785 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A024ECD2
• Locus tag: OU5_RS15785
• Protein ID: WP_010464623.1
• Coordinates: 3570148..3570378 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OU5_RS15745	3565464..3565736	+	273	WP_010464608.1	oxidative damage protection protein	-
OU5_RS15755	3566248..3566709	+	462	WP_010464610.1	DUF4234 domain-containing protein	-
OU5_RS15760	3566747..3567196	-	450	WP_010464612.1	hypothetical protein	-
OU5_RS15765	3567459..3567902	+	444	Protein_3144	hypothetical protein	-
OU5_RS15770	3568085..3569059	+	975	WP_010464616.1	alpha/beta fold hydrolase	-
OU5_RS15775	3569097..3569513	+	417	WP_010464618.1	hypothetical protein	-
OU5_RS15780	3569519..3569971	+	453	WP_010464620.1	hypothetical protein	-
OU5_RS15785	3570148..3570378	+	231	WP_010464623.1	antitoxin	Antitoxin
OU5_RS15790	3570378..3570785	+	408	WP_010464625.1	tRNA(fMet)-specific endonuclease VapC	Toxin
OU5_RS15795	3570867..3571193	-	327	WP_010464627.1	hypothetical protein	-
OU5_RS15800	3571539..3572930	-	1392	WP_010464628.1	GABA permease	-
OU5_RS15805	3573483..3574256	+	774	WP_007897462.1	ABC transporter ATP-binding protein	-
OU5_RS15810	3574270..3575016	+	747	WP_010464636.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15067.44 Da
• Isoelectric Point: 6.0845

>T45138 WP_010464625.1 NZ_CP005960:3570378-3570785 [Pseudomonas mandelii JR-1]
MIKFMLDTNICIFTIKNKPQIVREAFNLHDGQLCISAITLMELIYGAEKSATPEKNLAVIEGFTARLEVFPFDNEAAAHT
GMIRSELAKAGTPIGPYDQMIAGHARSRGLIVVTNNLREFERVPGLRVEDWVHPD

Nucleotide

• Length: 408 bp

>T45138 NZ_CP005960:3570378-3570785 [Pseudomonas mandelii JR-1]
ATGATCAAGTTCATGCTCGATACCAATATCTGTATTTTCACCATCAAGAACAAACCGCAAATAGTCAGAGAAGCTTTCAA
CTTGCATGACGGACAGTTATGCATCAGCGCGATTACGCTGATGGAGTTGATCTACGGTGCCGAAAAATCAGCGACCCCCG
AAAAGAACCTCGCGGTAATTGAAGGCTTCACAGCCCGCCTTGAAGTTTTCCCTTTCGACAATGAAGCAGCCGCGCATACC
GGAATGATACGGTCCGAGCTAGCGAAGGCCGGAACGCCCATTGGTCCCTATGACCAAATGATTGCCGGACATGCGCGCTC
ACGAGGGTTAATTGTCGTCACCAACAATCTGCGTGAGTTCGAACGGGTGCCAGGATTACGTGTAGAGGATTGGGTTCATC
CCGATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8666.70 Da
• Isoelectric Point: 4.6544

>AT45138 WP_010464623.1 NZ_CP005960:3570148-3570378 [Pseudomonas mandelii JR-1]
MEQTTLFMSNRSQAVRLPKAVAMPSDVKRVNVFAIGRARIITPADETWDSWFDGDNVSTDFMTDRQQPAAQERESF

Nucleotide

• Length: 231 bp

>AT45138 NZ_CP005960:3570148-3570378 [Pseudomonas mandelii JR-1]
ATGGAACAGACTACCCTTTTCATGAGCAATCGAAGCCAGGCTGTCCGTCTGCCGAAAGCTGTGGCGATGCCAAGTGACGT
CAAACGGGTCAACGTATTTGCCATCGGTCGGGCTCGCATCATTACGCCTGCAGACGAGACATGGGATAGCTGGTTCGATG
GAGATAACGTCAGCACAGATTTTATGACTGATCGCCAACAACCCGCCGCTCAGGAGCGTGAGTCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A024ECD2