Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 116271..116796 Replicon plasmid p1ColV5155
Accession NZ_CP005931
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag L282_RS25425 Protein ID WP_001159871.1
Coordinates 116491..116796 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag L282_RS25420 Protein ID WP_000813630.1
Coordinates 116271..116489 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS25390 111319..112452 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
L282_RS25395 112486..112731 - 246 Protein_104 hypothetical protein -
L282_RS25400 112786..113463 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
L282_RS25405 113463..113810 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
L282_RS25410 113830..115401 + 1572 WP_000381395.1 IS66 family transposase -
L282_RS29405 115460..115717 + 258 WP_040111990.1 hypothetical protein -
L282_RS25420 116271..116489 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L282_RS25425 116491..116796 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
L282_RS25430 116797..117603 + 807 WP_000016968.1 site-specific integrase -
L282_RS25435 118283..119014 - 732 WP_000504262.1 replication initiation protein -
L282_RS25440 119636..120841 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / vat 1..194170 194170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T45131 WP_001159871.1 NZ_CP005931:116491-116796 [Escherichia coli APEC IMT5155]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T45131 NZ_CP005931:116491-116796 [Escherichia coli APEC IMT5155]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT45131 WP_000813630.1 NZ_CP005931:116271-116489 [Escherichia coli APEC IMT5155]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT45131 NZ_CP005931:116271-116489 [Escherichia coli APEC IMT5155]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References