Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 108958..109601 Replicon plasmid p1ColV5155
Accession NZ_CP005931
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag L282_RS25370 Protein ID WP_001034046.1
Coordinates 108958..109374 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag L282_RS25375 Protein ID WP_001261278.1
Coordinates 109371..109601 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS25355 104163..104579 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
L282_RS25360 104576..104806 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
L282_RS25365 105119..108913 + 3795 WP_001144732.1 hypothetical protein -
L282_RS25370 108958..109374 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
L282_RS25375 109371..109601 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
L282_RS25380 109866..110366 + 501 WP_000528932.1 hypothetical protein -
L282_RS25385 110379..111152 + 774 WP_000905949.1 hypothetical protein -
L282_RS25390 111319..112452 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
L282_RS25395 112486..112731 - 246 Protein_104 hypothetical protein -
L282_RS25400 112786..113463 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
L282_RS25405 113463..113810 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / vat 1..194170 194170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T45130 WP_001034046.1 NZ_CP005931:c109374-108958 [Escherichia coli APEC IMT5155]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T45130 NZ_CP005931:c109374-108958 [Escherichia coli APEC IMT5155]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT45130 WP_001261278.1 NZ_CP005931:c109601-109371 [Escherichia coli APEC IMT5155]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT45130 NZ_CP005931:c109601-109371 [Escherichia coli APEC IMT5155]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References