Detailed information of TA system TA045126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 31049..31584
• Replicon: plasmid p1ColV5155
• Accession: NZ_CP005931
• Organism: Escherichia coli APEC IMT5155

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: L282_RS24965
• Protein ID: WP_000222760.1
• Coordinates: 31049..31336 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: L282_RS24970
• Protein ID: WP_001132900.1
• Coordinates: 31333..31584 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L282_RS24930	26633..27953	-	1321	Protein_25	ISL3 family transposase	-
L282_RS24935	27953..28317	+	365	Protein_26	IS1 family transposase	-
L282_RS24945	28372..29154	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
L282_RS24950	29151..30173	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
L282_RS24955	30262..30609	+	348	Protein_29	IS1-like element IS1A family transposase	-
L282_RS24965	31049..31336	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L282_RS24970	31333..31584	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L282_RS24980	32547..33404	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
L282_RS24985	33397..33879	-	483	WP_001273588.1	hypothetical protein	-
L282_RS24990	33872..33946	-	75	WP_001442103.1	RepA leader peptide Tap	-
L282_RS24995	34178..34435	-	258	WP_000083845.1	replication regulatory protein RepA	-
L282_RS25000	34719..34925	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	34912..34973	+	62	NuclAT_1	-	-
-	34912..34973	+	62	NuclAT_1	-	-
-	34912..34973	+	62	NuclAT_1	-	-
-	34912..34973	+	62	NuclAT_1	-	-
L282_RS29535	35229..35303	-	75	Protein_37	endonuclease	-
L282_RS25005	35549..35761	-	213	WP_040111969.1	ANR family transcriptional regulator	-
L282_RS25010	35897..36457	-	561	WP_040111971.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / vat	1..194170	194170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T45126 WP_000222760.1 NZ_CP005931:c31336-31049 [Escherichia coli APEC IMT5155]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T45126 NZ_CP005931:c31336-31049 [Escherichia coli APEC IMT5155]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT45126 WP_001132900.1 NZ_CP005931:c31584-31333 [Escherichia coli APEC IMT5155]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT45126 NZ_CP005931:c31584-31333 [Escherichia coli APEC IMT5155]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2