Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2543508..2544340 Replicon chromosome
Accession NZ_CP005930
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag L282_RS12570 Protein ID WP_000854753.1
Coordinates 2543508..2543882 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag L282_RS12575 Protein ID WP_001278232.1
Coordinates 2543972..2544340 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS12540 2539698..2540135 - 438 Protein_2353 DUF4942 domain-containing protein -
L282_RS12545 2540230..2540610 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
L282_RS12550 2540607..2540954 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
L282_RS12555 2541004..2542542 + 1539 WP_000998059.1 IS66 family transposase -
L282_RS29470 2542580..2542717 - 138 Protein_2357 restriction endonuclease subunit M -
L282_RS12560 2542814..2543011 - 198 WP_000839281.1 DUF957 domain-containing protein -
L282_RS12565 2543023..2543511 - 489 WP_000777541.1 hypothetical protein -
L282_RS12570 2543508..2543882 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
L282_RS12575 2543972..2544340 - 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
L282_RS12585 2544503..2544724 - 222 WP_000692303.1 DUF987 domain-containing protein -
L282_RS12590 2544787..2545263 - 477 WP_001186710.1 RadC family protein -
L282_RS12595 2545275..2545754 - 480 WP_000860065.1 antirestriction protein -
L282_RS12600 2545836..2546654 - 819 WP_001234631.1 DUF945 domain-containing protein -
L282_RS12605 2546753..2546986 - 234 WP_001278287.1 DUF905 family protein -
L282_RS12610 2546992..2547669 - 678 WP_001097306.1 hypothetical protein -
L282_RS12615 2547817..2548497 - 681 WP_001593697.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T45109 WP_000854753.1 NZ_CP005930:c2543882-2543508 [Escherichia coli APEC IMT5155]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T45109 NZ_CP005930:c2543882-2543508 [Escherichia coli APEC IMT5155]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT45109 WP_001278232.1 NZ_CP005930:c2544340-2543972 [Escherichia coli APEC IMT5155]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT45109 NZ_CP005930:c2544340-2543972 [Escherichia coli APEC IMT5155]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References