Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 43733..44354 Replicon plasmid pMI3
Accession NZ_CP005192
Organism Sphingobium sp. MI1205

Toxin (Protein)


Gene name shpA Uniprot ID M4S8P5
Locus tag K663_RS22515 Protein ID WP_007682056.1
Coordinates 43733..44041 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID T0GLH9
Locus tag K663_RS22520 Protein ID WP_007682049.1
Coordinates 44022..44354 (+) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K663_RS22480 38759..39013 + 255 WP_007682112.1 hypothetical protein -
K663_RS22485 39099..39989 + 891 WP_007682110.1 haloalkane dehalogenase -
K663_RS22490 40149..40913 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
K663_RS22495 40942..41196 + 255 WP_007682112.1 hypothetical protein -
K663_RS22500 41282..42172 + 891 WP_007682110.1 haloalkane dehalogenase -
K663_RS22505 42332..43096 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
K663_RS22510 43141..43701 + 561 WP_029987071.1 recombinase family protein -
K663_RS22515 43733..44041 + 309 WP_007682056.1 BrnT family toxin Toxin
K663_RS22520 44022..44354 + 333 WP_007682049.1 BrnA antitoxin family protein Antitoxin
K663_RS22525 44816..45490 - 675 WP_007682041.1 IS5 family transposase -
K663_RS24505 45673..45966 + 294 WP_145907151.1 hypothetical protein -
K663_RS22530 45911..46579 - 669 WP_081440685.1 EthD domain-containing protein -
K663_RS22535 46625..47386 - 762 WP_007682039.1 SDR family oxidoreductase -
K663_RS24175 47433..47594 - 162 WP_145902397.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88374 88374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(8-93)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(19-99)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11771.19 Da        Isoelectric Point: 6.2230

>T45083 WP_007682056.1 NZ_CP005192:43733-44041 [Sphingobium sp. MI1205]
MLSMRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEI
IHIISARKADRKERRRYEEERR

Download         Length: 309 bp

>T45083 NZ_CP005192:43733-44041 [Sphingobium sp. MI1205]
ATGTTATCCATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGC
CGCCAGCGCCTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGG
GCCTGGTCGAAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATC
ATCCATATCATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12476.26 Da        Isoelectric Point: 9.8217

>AT45083 WP_007682049.1 NZ_CP005192:44022-44354 [Sphingobium sp. MI1205]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Download         Length: 333 bp

>AT45083 NZ_CP005192:44022-44354 [Sphingobium sp. MI1205]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126RWR1

Antitoxin

Source ID Structure
AlphaFold DB T0GLH9

References