Detailed information of TA system TA045081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 87982..88658
• Replicon: plasmid pMI2
• Accession: NZ_CP005191
• Organism: Sphingobium sp. MI1205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K663_RS21220
• Protein ID: WP_020818771.1
• Coordinates: 87982..88395 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: T0J953
• Locus tag: K663_RS21225
• Protein ID: WP_020818770.1
• Coordinates: 88392..88658 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K663_RS21175	83102..83386	-	285	WP_015460590.1	hypothetical protein	-
K663_RS21180	83398..84093	-	696	WP_015460589.1	hypothetical protein	-
K663_RS21190	84406..85077	+	672	WP_020818773.1	hypothetical protein	-
K663_RS21195	85070..85942	+	873	WP_015460587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
K663_RS21200	86082..86387	-	306	WP_015460586.1	hypothetical protein	-
K663_RS21205	86392..86781	-	390	WP_015460585.1	hypothetical protein	-
K663_RS21210	86785..87258	-	474	WP_015460584.1	hypothetical protein	-
K663_RS21215	87255..87773	-	519	WP_020818772.1	hypothetical protein	-
K663_RS21220	87982..88395	-	414	WP_020818771.1	type II toxin-antitoxin system VapC family toxin	Toxin
K663_RS21225	88392..88658	-	267	WP_020818770.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K663_RS21230	89001..90464	+	1464	WP_167331044.1	IS21 family transposase	-
K663_RS21235	90464..91273	+	810	WP_020818758.1	IS21-like element helper ATPase IstB	-
K663_RS21240	91491..91853	-	363	WP_062121841.1	dioxygenase	-
K663_RS21245	91919..92347	-	429	WP_021222964.1	DoxX family protein	-
K663_RS21250	92564..93502	-	939	WP_020818768.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..287488	287488
	-	flank	IS/Tn	-	-	90464..91273	809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (17-54)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14585.68 Da
• Isoelectric Point: 4.4056

>T45081 WP_020818771.1 NZ_CP005191:c88395-87982 [Sphingobium sp. MI1205]
VSGLYMLDTNTVSELARNPQGSVAARIAEVGPDAICVSIITAAELRYGCARAGSPRLLAQIEAILDSLQILALDVPADTE
YAGIRAELEAAGKPIGPNDWFIAAHAYALEAVLVTANITDFSRIRALKVENWIADLH

Nucleotide

• Length: 414 bp

>T45081 NZ_CP005191:c88395-87982 [Sphingobium sp. MI1205]
GTGAGCGGGCTCTATATGCTCGACACCAATACCGTCTCCGAGCTTGCCCGCAATCCGCAAGGATCGGTTGCCGCACGCAT
CGCCGAAGTGGGCCCGGATGCGATCTGTGTCAGCATCATCACCGCGGCGGAACTCCGCTACGGCTGCGCCAGAGCGGGTT
CGCCGCGGCTGCTGGCGCAGATCGAGGCCATTCTGGACAGCCTCCAGATCCTTGCCCTCGATGTCCCCGCCGACACTGAA
TATGCCGGTATTCGCGCAGAGCTGGAAGCAGCCGGCAAGCCTATCGGGCCAAATGACTGGTTCATCGCTGCCCATGCCTA
TGCCCTGGAAGCGGTGCTGGTGACCGCGAATATCACAGACTTCTCGCGGATACGCGCACTCAAAGTCGAGAACTGGATCG
CCGACCTTCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9978.64 Da
• Isoelectric Point: 8.7675

>AT45081 WP_020818770.1 NZ_CP005191:c88658-88392 [Sphingobium sp. MI1205]
MARKKAVSVPPREAKLFRNNKSQALRIPADFELPGDRVMIHRDGDRLIIEPVRRKNLLEVLASLQPLGPDDQFPDVEDTL
LPIKAIDL

Nucleotide

• Length: 267 bp

>AT45081 NZ_CP005191:c88658-88392 [Sphingobium sp. MI1205]
ATGGCCCGGAAAAAAGCAGTTTCCGTCCCTCCACGAGAAGCCAAACTGTTCCGGAACAACAAGAGTCAGGCTCTGCGCAT
CCCTGCCGATTTCGAACTTCCGGGTGACCGCGTGATGATCCATCGCGATGGCGACCGCCTCATTATCGAACCGGTACGCC
GCAAGAATCTTCTGGAAGTCCTCGCATCCCTGCAGCCGCTCGGGCCCGATGATCAGTTTCCCGATGTCGAGGACACATTG
CTCCCGATAAAGGCCATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A126RG80