Detailed information of TA system TA045080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 14813..15447
• Replicon: plasmid pMI2
• Accession: NZ_CP005191
• Organism: Sphingobium sp. MI1205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: T0GCG3
• Locus tag: K663_RS20765
• Protein ID: WP_007682494.1
• Coordinates: 14813..15202 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: T0GE84
• Locus tag: K663_RS20770
• Protein ID: WP_007682496.1
• Coordinates: 15199..15447 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K663_RS20730	10127..10679	+	553	Protein_18	IS21 family transposase	-
K663_RS20735	10676..11539	+	864	WP_004211466.1	IS21-like element helper ATPase IstB	-
K663_RS20740	11621..11944	+	324	Protein_20	transposase	-
K663_RS20745	11990..13087	-	1098	WP_007683624.1	IS630 family transposase	-
K663_RS20750	13151..14113	-	963	WP_007682488.1	tyrosine-type recombinase/integrase	-
K663_RS20755	14113..14511	-	399	WP_007682491.1	hypothetical protein	-
K663_RS20760	14508..14813	-	306	WP_007682492.1	hypothetical protein	-
K663_RS20765	14813..15202	-	390	WP_007682494.1	type II toxin-antitoxin system VapC family toxin	Toxin
K663_RS20770	15199..15447	-	249	WP_007682496.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K663_RS20775	15616..17019	-	1404	WP_013038665.1	efflux transporter outer membrane subunit	-
K663_RS20780	17012..18850	-	1839	WP_021246208.1	efflux RND transporter permease subunit	-
K663_RS20785	18879..19643	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K663_RS20790	19695..20003	+	309	Protein_30	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..287488	287488
	-	flank	IS/Tn	-	-	18879..19643	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-123)

Antitoxin

• Antitoxin VapB-like (1-81)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14083.02 Da
• Isoelectric Point: 4.5934

>T45080 WP_007682494.1 NZ_CP005191:c15202-14813 [Sphingobium sp. MI1205]
MILDTSALVAILYREPEAATFAQAIHDADACRISVASYVELSMVIERQLGPEGMRQTEAFFRRAGITIEPVTLDHGELAR
QAFLDFGKGRHKAGLNFGDCFSYALAKATDEPLLFKGNDFALTDIEAAA

Nucleotide

• Length: 390 bp

>T45080 NZ_CP005191:c15202-14813 [Sphingobium sp. MI1205]
ATGATCCTCGACACCTCGGCCCTGGTCGCGATCCTCTATCGCGAACCCGAAGCGGCGACCTTCGCACAAGCCATCCATGA
TGCCGATGCCTGCCGCATCAGCGTCGCCAGCTATGTCGAGCTGTCGATGGTCATCGAACGCCAGCTCGGTCCCGAAGGGA
TGCGCCAGACCGAAGCCTTCTTTCGCCGCGCCGGCATCACGATCGAACCGGTGACGCTCGATCATGGCGAACTGGCGCGA
CAGGCGTTTCTCGATTTCGGCAAGGGCCGCCACAAAGCGGGCCTGAACTTTGGCGACTGCTTTTCCTATGCGCTGGCCAA
GGCGACCGACGAGCCGCTGTTGTTCAAGGGCAATGATTTTGCCCTGACGGACATAGAGGCGGCGGCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9203.47 Da
• Isoelectric Point: 9.7320

>AT45080 WP_007682496.1 NZ_CP005191:c15447-15199 [Sphingobium sp. MI1205]
MSLNVKDPEAHRLAQAIAQATGQSMTRVVTEALRERFARIERQKSKASVAELLTIADRAATHVKRPYVDHAELFYDDNGL
PK

Nucleotide

• Length: 249 bp

>AT45080 NZ_CP005191:c15447-15199 [Sphingobium sp. MI1205]
ATGAGCCTCAATGTTAAAGACCCTGAAGCCCATCGGCTTGCGCAGGCCATCGCCCAAGCGACCGGCCAGAGCATGACCCG
CGTGGTGACGGAGGCGCTGCGCGAGCGCTTCGCCAGGATCGAGCGTCAGAAAAGCAAGGCGAGCGTCGCGGAACTGCTAA
CGATCGCCGATCGCGCCGCCACGCATGTGAAGCGCCCCTATGTCGATCATGCCGAACTGTTTTATGACGATAATGGCCTG
CCGAAATGA

Structures

Toxin

• AlphaFold DB: T0GCG3

Antitoxin

• AlphaFold DB: T0GE84