Detailed information of TA system TA045079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 176838..177459
• Replicon: plasmid pMI1
• Accession: NZ_CP005190
• Organism: Sphingobium sp. MI1205

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M4S8P5
• Locus tag: K663_RS20055
• Protein ID: WP_007682056.1
• Coordinates: 177151..177459 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: T0GLH9
• Locus tag: K663_RS20050
• Protein ID: WP_007682049.1
• Coordinates: 176838..177170 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K663_RS20025	171968..172732	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K663_RS20030	172785..173759	+	975	Protein_171	Rieske 2Fe-2S domain-containing protein	-
K663_RS20035	173806..174567	+	762	WP_007682039.1	SDR family oxidoreductase	-
K663_RS20040	174613..175281	+	669	WP_081440685.1	EthD domain-containing protein	-
K663_RS24480	175226..175519	-	294	WP_145907151.1	hypothetical protein	-
K663_RS20045	175702..176376	+	675	WP_007682041.1	IS5 family transposase	-
K663_RS20050	176838..177170	-	333	WP_007682049.1	BrnA antitoxin family protein	Antitoxin
K663_RS20055	177151..177459	-	309	WP_007682056.1	BrnT family toxin	Toxin
K663_RS20060	177491..178051	-	561	WP_029987071.1	recombinase family protein	-
K663_RS20065	178096..178860	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K663_RS20070	179020..179910	-	891	WP_062121713.1	haloalkane dehalogenase	-
K663_RS20075	179996..180250	-	255	WP_007682112.1	hypothetical protein	-
K663_RS20080	180279..181043	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K663_RS23535	181088..181501	-	414	Protein_183	phytanoyl-CoA dioxygenase family protein	-
K663_RS20090	181501..182109	-	609	WP_062121811.1	autoinducer synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..292135	292135
	-	inside	IScluster/Tn	-	-	164592..185321	20729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-93)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-99)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11771.19 Da
• Isoelectric Point: 6.2230

>T45079 WP_007682056.1 NZ_CP005190:c177459-177151 [Sphingobium sp. MI1205]
MLSMRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEI
IHIISARKADRKERRRYEEERR

Nucleotide

• Length: 309 bp

>T45079 NZ_CP005190:c177459-177151 [Sphingobium sp. MI1205]
ATGTTATCCATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGC
CGCCAGCGCCTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGG
GCCTGGTCGAAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATC
ATCCATATCATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12476.26 Da
• Isoelectric Point: 9.8217

>AT45079 WP_007682049.1 NZ_CP005190:c177170-176838 [Sphingobium sp. MI1205]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Nucleotide

• Length: 333 bp

>AT45079 NZ_CP005190:c177170-176838 [Sphingobium sp. MI1205]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Structures

Toxin

• AlphaFold DB: A0A126RWR1

Antitoxin

• AlphaFold DB: T0GLH9