Detailed information of TA system TA045077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1970285..1970946
• Replicon: chromosome
• Accession: NZ_CP005188
• Organism: Sphingobium sp. MI1205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A126R8B2
• Locus tag: K663_RS09460
• Protein ID: WP_062116594.1
• Coordinates: 1970509..1970946 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K663_RS09455
• Protein ID: WP_062116589.1
• Coordinates: 1970285..1970512 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K663_RS09425	1965758..1966771	+	1014	WP_062116575.1	FecR domain-containing protein	-
K663_RS09430	1966732..1967466	-	735	WP_062116577.1	NAD-dependent deacylase	-
K663_RS09435	1967629..1968393	+	765	WP_062116580.1	4-hydroxy-tetrahydrodipicolinate reductase	-
K663_RS09440	1968401..1969060	+	660	WP_062116583.1	endonuclease III	-
K663_RS09445	1969167..1969553	+	387	WP_062116586.1	hypothetical protein	-
K663_RS09455	1970285..1970512	+	228	WP_062116589.1	hypothetical protein	Antitoxin
K663_RS09460	1970509..1970946	+	438	WP_062116594.1	PIN domain-containing protein	Toxin
K663_RS09465	1970981..1972210	+	1230	WP_083535862.1	protein phosphatase 2C domain-containing protein	-
K663_RS09470	1972207..1973277	+	1071	WP_062116600.1	serine/threonine protein kinase	-
K663_RS09475	1973274..1974347	+	1074	WP_062116603.1	serine/threonine protein kinase	-
K663_RS09480	1974349..1975221	-	873	WP_062116606.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15301.47 Da
• Isoelectric Point: 6.8370

>T45077 WP_062116594.1 NZ_CP005188:1970509-1970946 [Sphingobium sp. MI1205]
VTFLLDVNVLIALIDPAHISHDPAHQWFESVGKVSWATCPITENGVIRIVGHASYPNTPGSTADVAAIMAKMCGLSGHAF
WPDSLSLTDAAHVDASQVLTSAQVTDTYLLALARANGGKLATFDRRLSVKAVKGGKETLHIIGNS

Nucleotide

• Length: 438 bp

>T45077 NZ_CP005188:1970509-1970946 [Sphingobium sp. MI1205]
GTGACCTTTCTGCTCGACGTCAACGTACTCATCGCCCTGATCGATCCGGCACACATCAGCCATGATCCCGCGCACCAATG
GTTCGAGTCGGTTGGCAAGGTGTCCTGGGCGACCTGCCCCATAACGGAGAACGGGGTCATCCGCATCGTTGGCCATGCCA
GCTATCCCAATACACCGGGATCGACGGCAGATGTCGCGGCTATTATGGCCAAGATGTGCGGATTGTCGGGACACGCTTTC
TGGCCCGATAGCTTGAGCCTGACCGATGCTGCTCACGTCGATGCGTCGCAAGTGCTAACCTCGGCTCAAGTGACCGATAC
CTATTTGCTAGCGCTGGCCCGCGCCAATGGAGGAAAGCTAGCGACATTTGATAGACGCCTTTCGGTAAAGGCGGTGAAGG
GTGGAAAGGAAACGCTCCACATCATCGGTAATTCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8160.39 Da
• Isoelectric Point: 9.1095

>AT45077 WP_062116589.1 NZ_CP005188:1970285-1970512 [Sphingobium sp. MI1205]
MRTTLAIDDDVLVAAKAIARQQRRSVGEVVSDLARRALRRPQAPAARNGIPLLSVSDEATVVTLETVNLLRDELP

Nucleotide

• Length: 228 bp

>AT45077 NZ_CP005188:1970285-1970512 [Sphingobium sp. MI1205]
ATGCGAACCACATTGGCGATCGATGACGACGTATTAGTCGCGGCTAAGGCGATCGCGCGGCAGCAGCGCAGGAGTGTCGG
AGAGGTGGTGTCCGATCTCGCGCGCCGCGCATTGCGGCGCCCGCAAGCTCCCGCAGCGCGCAATGGCATTCCTCTTCTGT
CGGTATCGGATGAGGCAACGGTGGTCACGCTTGAAACGGTGAACTTGCTCCGCGACGAGCTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A126R8B2