Detailed information of TA system TA045075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 25860..26494
• Replicon: plasmid pTK4
• Accession: NZ_CP005088
• Organism: Sphingobium sp. TKS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: T0GCG3
• Locus tag: K426_RS29060
• Protein ID: WP_007682494.1
• Coordinates: 26105..26494 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: T0GE84
• Locus tag: K426_RS29055
• Protein ID: WP_007682496.1
• Coordinates: 25860..26108 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K426_RS31695	21486..21848	+	363	Protein_24	TonB-dependent receptor	-
K426_RS29030	21888..22640	+	753	WP_015460611.1	SDR family oxidoreductase	-
K426_RS29035	22662..23348	+	687	WP_007682394.1	EthD domain-containing protein	-
K426_RS29040	23348..23710	+	363	WP_007682395.1	hypothetical protein	-
K426_RS29045	23808..24572	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K426_RS29050	24634..25691	+	1058	Protein_29	efflux transporter outer membrane subunit	-
K426_RS29055	25860..26108	+	249	WP_007682496.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K426_RS29060	26105..26494	+	390	WP_007682494.1	type II toxin-antitoxin system VapC family toxin	Toxin
K426_RS29065	26494..26799	+	306	WP_007682492.1	hypothetical protein	-
K426_RS29070	26796..27194	+	399	WP_007682491.1	hypothetical protein	-
K426_RS29075	27194..28156	+	963	WP_007682488.1	tyrosine-type recombinase/integrase	-
K426_RS29080	28220..29317	+	1098	WP_007683624.1	IS630 family transposase	-
K426_RS29085	29363..29686	-	324	Protein_36	transposase	-
K426_RS29090	29768..30631	-	864	WP_004211466.1	IS21-like element helper ATPase IstB	-
K426_RS29095	30628..31180	-	553	Protein_38	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..75938	75938
	-	inside	IScluster/Tn	-	-	20658..37220	16562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-123)

Antitoxin

• Antitoxin VapB-like (1-81)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14083.02 Da
• Isoelectric Point: 4.5934

>T45075 WP_007682494.1 NZ_CP005088:26105-26494 [Sphingobium sp. TKS]
MILDTSALVAILYREPEAATFAQAIHDADACRISVASYVELSMVIERQLGPEGMRQTEAFFRRAGITIEPVTLDHGELAR
QAFLDFGKGRHKAGLNFGDCFSYALAKATDEPLLFKGNDFALTDIEAAA

Nucleotide

• Length: 390 bp

>T45075 NZ_CP005088:26105-26494 [Sphingobium sp. TKS]
ATGATCCTCGACACCTCGGCCCTGGTCGCGATCCTCTATCGCGAACCCGAAGCGGCGACCTTCGCACAAGCCATCCATGA
TGCCGATGCCTGCCGCATCAGCGTCGCCAGCTATGTCGAGCTGTCGATGGTCATCGAACGCCAGCTCGGTCCCGAAGGGA
TGCGCCAGACCGAAGCCTTCTTTCGCCGCGCCGGCATCACGATCGAACCGGTGACGCTCGATCATGGCGAACTGGCGCGA
CAGGCGTTTCTCGATTTCGGCAAGGGCCGCCACAAAGCGGGCCTGAACTTTGGCGACTGCTTTTCCTATGCGCTGGCCAA
GGCGACCGACGAGCCGCTGTTGTTCAAGGGCAATGATTTTGCCCTGACGGACATAGAGGCGGCGGCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9203.47 Da
• Isoelectric Point: 9.7320

>AT45075 WP_007682496.1 NZ_CP005088:25860-26108 [Sphingobium sp. TKS]
MSLNVKDPEAHRLAQAIAQATGQSMTRVVTEALRERFARIERQKSKASVAELLTIADRAATHVKRPYVDHAELFYDDNGL
PK

Nucleotide

• Length: 249 bp

>AT45075 NZ_CP005088:25860-26108 [Sphingobium sp. TKS]
ATGAGCCTCAATGTTAAAGACCCTGAAGCCCATCGGCTTGCGCAGGCCATCGCCCAAGCGACCGGCCAGAGCATGACCCG
CGTGGTGACGGAGGCGCTGCGCGAGCGCTTCGCCAGGATCGAGCGTCAGAAAAGCAAGGCGAGCGTCGCGGAACTGCTAA
CGATCGCCGATCGCGCCGCCACGCATGTGAAGCGCCCCTATGTCGATCATGCCGAACTGTTTTATGACGATAATGGCCTG
CCGAAATGA

Structures

Toxin

• AlphaFold DB: T0GCG3

Antitoxin

• AlphaFold DB: T0GE84