Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 37403..38024 Replicon plasmid pTK3
Accession NZ_CP005087
Organism Sphingobium sp. TKS

Toxin (Protein)


Gene name shpA Uniprot ID M4S8P5
Locus tag K426_RS28640 Protein ID WP_007682056.1
Coordinates 37403..37711 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID T0GLH9
Locus tag K426_RS28645 Protein ID WP_007682049.1
Coordinates 37692..38024 (+) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K426_RS28610 32687..33409 + 723 WP_015460630.1 hypothetical protein -
K426_RS28615 33819..34583 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
K426_RS28620 34612..34866 + 255 WP_007682112.1 hypothetical protein -
K426_RS28625 34952..35842 + 891 WP_066564812.1 haloalkane dehalogenase -
K426_RS28630 36002..36766 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
K426_RS28635 36811..37371 + 561 WP_029987071.1 recombinase family protein -
K426_RS28640 37403..37711 + 309 WP_007682056.1 BrnT family toxin Toxin
K426_RS28645 37692..38024 + 333 WP_007682049.1 BrnA antitoxin family protein Antitoxin
K426_RS28650 38486..39181 - 696 Protein_42 IS5 family transposase -
K426_RS31980 39343..39636 + 294 WP_145907151.1 hypothetical protein -
K426_RS28655 39581..40249 - 669 WP_081440685.1 EthD domain-containing protein -
K426_RS28660 40295..41056 - 762 WP_007682039.1 SDR family oxidoreductase -
K426_RS28665 41103..42077 - 975 Protein_46 Rieske 2Fe-2S domain-containing protein -
K426_RS28670 42130..42894 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..87635 87635
- inside IScluster/Tn - - 33819..42894 9075


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(8-93)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(19-99)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11771.19 Da        Isoelectric Point: 6.2230

>T45074 WP_007682056.1 NZ_CP005087:37403-37711 [Sphingobium sp. TKS]
MLSMRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEI
IHIISARKADRKERRRYEEERR

Download         Length: 309 bp

>T45074 NZ_CP005087:37403-37711 [Sphingobium sp. TKS]
ATGTTATCCATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGC
CGCCAGCGCCTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGG
GCCTGGTCGAAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATC
ATCCATATCATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12476.26 Da        Isoelectric Point: 9.8217

>AT45074 WP_007682049.1 NZ_CP005087:37692-38024 [Sphingobium sp. TKS]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Download         Length: 333 bp

>AT45074 NZ_CP005087:37692-38024 [Sphingobium sp. TKS]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126RWR1

Antitoxin

Source ID Structure
AlphaFold DB T0GLH9

References