Detailed information of TA system TA045073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 428092..428623
• Replicon: plasmid pTK1
• Accession: NZ_CP005085
• Organism: Sphingobium sp. TKS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: K426_RS27110
• Protein ID: WP_004212690.1
• Coordinates: 428092..428373 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: K426_RS27115
• Protein ID: WP_004212689.1
• Coordinates: 428360..428623 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K426_RS27085	424144..424617	+	474	WP_007683361.1	DUF305 domain-containing protein	-
K426_RS27090	424676..426211	+	1536	WP_007683359.1	PAS domain S-box protein	-
K426_RS27095	426201..426824	+	624	WP_007683357.1	response regulator transcription factor	-
K426_RS27100	426906..427313	+	408	WP_007683354.1	response regulator	-
K426_RS27105	427376..427726	-	351	WP_007683352.1	magnesium transporter	-
K426_RS27110	428092..428373	-	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K426_RS27115	428360..428623	-	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K426_RS27120	429008..429445	-	438	WP_007683347.1	metal-binding protein	-
K426_RS27125	429525..430766	-	1242	WP_007683345.1	MFS transporter	-
K426_RS27130	430760..431746	-	987	WP_009823975.1	copper resistance protein B	-
K426_RS27135	431743..433449	-	1707	WP_007683343.1	copper resistance system multicopper oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..520614	520614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T45073 WP_004212690.1 NZ_CP005085:c428373-428092 [Sphingobium sp. TKS]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T45073 NZ_CP005085:c428373-428092 [Sphingobium sp. TKS]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT45073 WP_004212689.1 NZ_CP005085:c428623-428360 [Sphingobium sp. TKS]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT45073 NZ_CP005085:c428623-428360 [Sphingobium sp. TKS]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968