Detailed information of TA system TA045071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 272978..273489
• Replicon: plasmid pTK1
• Accession: NZ_CP005085
• Organism: Sphingobium sp. TKS

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: T0GMU0
• Locus tag: K426_RS26495
• Protein ID: WP_021243652.1
• Coordinates: 273244..273489 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: T0I0K2
• Locus tag: K426_RS26490
• Protein ID: WP_021243651.1
• Coordinates: 272978..273247 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K426_RS26465	268488..268970	+	483	WP_021243646.1	hypothetical protein	-
K426_RS26470	269021..269725	-	705	WP_021243647.1	hypothetical protein	-
K426_RS26475	269722..269979	-	258	WP_066564206.1	hypothetical protein	-
K426_RS26480	270345..271871	+	1527	WP_031290542.1	Do family serine endopeptidase	-
K426_RS26485	272180..272449	+	270	WP_021243650.1	hypothetical protein	-
K426_RS26490	272978..273247	-	270	WP_021243651.1	BrnA antitoxin family protein	Antitoxin
K426_RS26495	273244..273489	-	246	WP_021243652.1	BrnT family toxin	Toxin
K426_RS26500	273827..274153	-	327	WP_021243653.1	hypothetical protein	-
K426_RS26505	274455..275171	-	717	WP_021243654.1	hypothetical protein	-
K426_RS26510	275476..276795	-	1320	WP_031290546.1	SLC13 family permease	-
K426_RS26515	276906..277751	+	846	WP_031290547.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..520614	520614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (16-86)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9051.48 Da
• Isoelectric Point: 10.2690

>T45071 WP_021243652.1 NZ_CP005085:c273489-273244 [Sphingobium sp. TKS]
MIIKWGEPKRQANIAKHRIDFADIGEEFFASALIGDAKDGRYFALGEWNGFIVVIFVRLGSEGVSIVSARPASRKERKLL
S

Nucleotide

• Length: 246 bp

>T45071 NZ_CP005085:c273489-273244 [Sphingobium sp. TKS]
ATGATCATCAAGTGGGGCGAACCGAAACGGCAAGCGAACATCGCCAAGCATCGCATCGATTTTGCTGACATCGGAGAAGA
GTTTTTCGCTTCCGCACTGATCGGCGACGCCAAGGATGGTCGGTACTTCGCGCTCGGCGAGTGGAACGGTTTCATCGTGG
TGATTTTTGTCAGGCTTGGCAGCGAGGGTGTTTCCATCGTTTCAGCCCGGCCCGCCAGTCGCAAGGAAAGGAAGCTGCTG
TCATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9726.07 Da
• Isoelectric Point: 9.8109

>AT45071 WP_021243651.1 NZ_CP005085:c273247-272978 [Sphingobium sp. TKS]
MTQAKYTQADMDAVSDNPELTAEDIAKAKPFAEAFPDLAATIRRRGVQKAPTKVSTTVRLDQDVLARLKADGPGWQTRLN
DTLRKALGV

Nucleotide

• Length: 270 bp

>AT45071 NZ_CP005085:c273247-272978 [Sphingobium sp. TKS]
ATGACTCAAGCCAAGTATACGCAGGCCGATATGGATGCGGTGTCGGACAACCCTGAATTGACGGCAGAGGATATCGCTAA
GGCCAAGCCCTTCGCTGAAGCCTTCCCGGATCTCGCTGCTACCATCCGTCGTCGGGGAGTACAGAAAGCCCCCACGAAGG
TGAGTACAACCGTTCGGTTGGATCAGGACGTATTGGCCCGGTTGAAGGCCGATGGACCGGGCTGGCAGACCCGCTTGAAT
GACACGCTCCGAAAAGCGTTGGGAGTATAG

Structures

Toxin

• AlphaFold DB: T0GMU0

Antitoxin

• AlphaFold DB: T0I0K2