Detailed information of TA system TA045065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 534184..535049
• Replicon: chromosome
• Accession: NZ_CP005083
• Organism: Sphingobium sp. TKS

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A126RHE8
• Locus tag: K426_RS02745
• Protein ID: WP_066553427.1
• Coordinates: 534184..534762 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: K426_RS32070
• Protein ID: WP_197672790.1
• Coordinates: 534759..535049 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K426_RS02715	529864..530271	+	408	WP_066553416.1	OB-fold domain-containing protein	-
K426_RS02720	530286..531281	+	996	WP_066553418.1	quinone oxidoreductase	-
K426_RS02725	531328..532170	+	843	WP_066553420.1	SDR family NAD(P)-dependent oxidoreductase	-
K426_RS31145	532552..532845	-	294	WP_145907154.1	transposase	-
K426_RS02735	533520..533798	-	279	Protein_544	chromosome partitioning protein ParB	-
K426_RS02740	533874..534119	+	246	WP_066553425.1	DUF3883 domain-containing protein	-
K426_RS02745	534184..534762	-	579	WP_066553427.1	PIN domain-containing protein	Toxin
K426_RS32070	534759..535049	-	291	WP_197672790.1	helix-turn-helix domain-containing protein	Antitoxin
K426_RS02755	535504..537369	-	1866	WP_066553431.1	group II intron reverse transcriptase/maturase	-
K426_RS31775	537996..538436	-	441	WP_066553439.1	hypothetical protein	-
K426_RS02765	538599..538838	+	240	WP_066553442.1	type II toxin-antitoxin system ParD family antitoxin	-
K426_RS02770	538835..539161	+	327	WP_066553445.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	539573..540796	1223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-116)

Antitoxin

• SinI-like, DNA-binding domain (27-73)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21650.68 Da
• Isoelectric Point: 4.4549

>T45065 WP_066553427.1 NZ_CP005083:c534762-534184 [Sphingobium sp. TKS]
MSDIADRFVVVLDANVLYPFRVRDALLRFAEAGLFRARWSPTIMDEWTRNLLDRRPDMEPSIQSQVAAMERAFPESCVSG
AEGLIPALSLPDPDDRHVLATAIRTDAEHIVTENLKDFPDEALAPFGITAVTPDDFLSSTFELYPGDALSTLRTMRQDYR
NPPFSPGEFIFELQRKGLPKLASMLKEHIDLL

Nucleotide

• Length: 579 bp

>T45065 NZ_CP005083:c534762-534184 [Sphingobium sp. TKS]
TTGAGCGACATAGCCGACAGATTTGTCGTCGTTCTCGACGCCAACGTCCTTTACCCCTTCCGGGTCAGGGATGCCCTGCT
TCGCTTCGCCGAAGCAGGGCTGTTCCGCGCGCGCTGGTCCCCGACCATCATGGACGAGTGGACGCGCAATCTGCTCGACA
GGCGGCCGGACATGGAGCCCAGCATTCAATCCCAGGTCGCTGCGATGGAACGCGCCTTTCCTGAAAGCTGCGTCAGCGGC
GCGGAAGGGCTCATCCCGGCGCTCAGCTTGCCCGACCCCGATGATCGCCATGTGCTGGCCACGGCCATCCGGACGGACGC
CGAGCATATCGTCACTGAAAATCTGAAGGATTTCCCGGACGAAGCACTCGCCCCGTTCGGCATCACCGCCGTGACGCCCG
ACGATTTCCTGAGCAGCACGTTCGAGCTTTATCCGGGGGACGCGCTGTCCACCCTGCGAACCATGCGGCAAGACTATAGA
AACCCACCATTTTCTCCCGGCGAGTTCATCTTCGAACTCCAGCGCAAAGGCCTGCCCAAATTGGCCTCCATGCTGAAGGA
GCATATCGATCTCCTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10796.39 Da
• Isoelectric Point: 6.8814

>AT45065 WP_197672790.1 NZ_CP005083:c535049-534759 [Sphingobium sp. TKS]
MGQLMLDVLTHVGRGEMITLVPYGAELTTKEAADLLNVSRPFLVKLLDEGVIGHHKVGSHRRIRASELLAYKARRDEERR
SALSELQRLGQEFDAD

Nucleotide

• Length: 291 bp

>AT45065 NZ_CP005083:c535049-534759 [Sphingobium sp. TKS]
CTGGGACAGCTCATGCTCGACGTGCTGACCCATGTCGGTCGCGGCGAGATGATCACGCTCGTCCCCTATGGGGCGGAACT
CACCACGAAGGAGGCCGCCGATCTGCTGAACGTGTCTCGCCCCTTCCTAGTGAAGCTGCTCGACGAAGGGGTGATCGGCC
ATCACAAGGTCGGCTCCCACCGCAGGATAAGGGCGAGCGAACTGCTGGCCTACAAGGCCAGAAGGGACGAAGAGCGACGC
AGCGCGCTGAGCGAGCTGCAGCGTCTCGGCCAGGAGTTTGACGCCGATTGA

Structures

Toxin

• AlphaFold DB: A0A126RHE8