Detailed information of TA system TA045064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 338736..339412
• Replicon: chromosome
• Accession: NZ_CP005083
• Organism: Sphingobium sp. TKS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K426_RS01640
• Protein ID: WP_197672744.1
• Coordinates: 339014..339412 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: T0J953
• Locus tag: K426_RS01635
• Protein ID: WP_020818770.1
• Coordinates: 338736..339002 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K426_RS01615	333976..334830	+	855	WP_197672787.1	substrate binding domain-containing protein	-
K426_RS01620	335047..335475	+	429	WP_021222964.1	DoxX family protein	-
K426_RS30010	335541..335903	+	363	WP_062121841.1	dioxygenase	-
K426_RS01625	336121..336930	-	810	WP_020818758.1	IS21-like element helper ATPase IstB	-
K426_RS01630	336930..338393	-	1464	WP_167331044.1	IS21 family transposase	-
K426_RS01635	338736..339002	+	267	WP_020818770.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K426_RS01640	339014..339412	+	399	WP_197672744.1	type II toxin-antitoxin system VapC family toxin	Toxin
K426_RS01645	339621..340139	+	519	WP_020818772.1	hypothetical protein	-
K426_RS01650	340136..340609	+	474	WP_015460584.1	hypothetical protein	-
K426_RS01655	340613..341002	+	390	WP_015460585.1	hypothetical protein	-
K426_RS01660	341007..341312	+	306	WP_015460586.1	hypothetical protein	-
K426_RS01665	341452..342324	-	873	WP_015460587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
K426_RS01670	342317..342988	-	672	WP_020818773.1	hypothetical protein	-
K426_RS01680	343301..343996	+	696	WP_015460589.1	hypothetical protein	-
K426_RS01685	344008..344292	+	285	WP_015460590.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• SpoVT-AbrB domain (17-54)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14066.08 Da
• Isoelectric Point: 4.4056

>T45064 WP_197672744.1 NZ_CP005083:339014-339412 [Sphingobium sp. TKS]
MLDTNTVSELARNPQGSVAARIAEVGPDAICVSIITAAELRYGCARAGSPRLLAQIEAILDSLQILALDVPADTEYAGIR
AELEAAGKPIGPNDWFIAAHAYALEAVLVTANITDFSRIRALKVENWIADLH

Nucleotide

• Length: 399 bp

>T45064 NZ_CP005083:339014-339412 [Sphingobium sp. TKS]
ATGCTCGACACCAATACCGTCTCCGAGCTTGCCCGCAATCCGCAAGGATCGGTTGCCGCACGCATCGCCGAAGTGGGCCC
GGATGCGATCTGTGTCAGCATCATCACCGCGGCGGAACTCCGCTACGGCTGCGCCAGAGCGGGTTCGCCGCGGCTGCTGG
CGCAGATCGAGGCCATTCTGGACAGCCTCCAGATCCTTGCCCTCGATGTCCCCGCCGACACTGAATATGCCGGTATTCGC
GCAGAGCTGGAAGCAGCCGGCAAGCCTATCGGGCCAAATGACTGGTTCATCGCTGCCCATGCCTATGCCCTGGAAGCGGT
GCTGGTGACCGCGAATATCACAGACTTCTCGCGGATACGCGCACTCAAAGTCGAGAACTGGATCGCCGACCTTCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9978.64 Da
• Isoelectric Point: 8.7675

>AT45064 WP_020818770.1 NZ_CP005083:338736-339002 [Sphingobium sp. TKS]
MARKKAVSVPPREAKLFRNNKSQALRIPADFELPGDRVMIHRDGDRLIIEPVRRKNLLEVLASLQPLGPDDQFPDVEDTL
LPIKAIDL

Nucleotide

• Length: 267 bp

>AT45064 NZ_CP005083:338736-339002 [Sphingobium sp. TKS]
ATGGCCCGGAAAAAAGCAGTTTCCGTCCCTCCACGAGAAGCCAAACTGTTCCGGAACAACAAGAGTCAGGCTCTGCGCAT
CCCTGCCGATTTCGAACTTCCGGGTGACCGCGTGATGATCCATCGCGATGGCGACCGCCTCATTATCGAACCGGTACGCC
GCAAGAATCTTCTGGAAGTCCTCGCATCCCTGCAGCCGCTCGGGCCCGATGATCAGTTTCCCGATGTCGAGGACACATTG
CTCCCGATAAAGGCCATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A126RG80