Detailed information of TA system TA045062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5879518..5880094
• Replicon: chromosome
• Accession: NZ_CP004887
• Organism: Klebsiella michiganensis HKOPL1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: J415_RS28845
• Protein ID: WP_071881753.1
• Coordinates: 5879807..5880094 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: J415_RS27245
• Protein ID: WP_014227757.1
• Coordinates: 5879518..5879820 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J415_RS27230	5876356..5876691	+	336	WP_014227760.1	endoribonuclease SymE	-
J415_RS27235	5877145..5878056	+	912	WP_014227759.1	acetamidase/formamidase family protein	-
J415_RS27240	5878053..5879396	+	1344	WP_014227758.1	APC family permease	-
J415_RS27245	5879518..5879820	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
J415_RS28845	5879807..5880094	-	288	WP_071881753.1	BrnT family toxin	Toxin
J415_RS27255	5880345..5880788	-	444	WP_014227755.1	FosA family fosfomycin resistance glutathione transferase	-
J415_RS27260	5880782..5881691	-	910	Protein_5425	LysR family transcriptional regulator	-
J415_RS27265	5881779..5882561	+	783	WP_014227753.1	NAD(P)H-dependent oxidoreductase	-
J415_RS27270	5882709..5883293	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
J415_RS27275	5883439..5884239	+	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
J415_RS27280	5884236..5884754	+	519	WP_004098240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T45062 WP_071881753.1 NZ_CP004887:c5880094-5879807 [Klebsiella michiganensis HKOPL1]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T45062 NZ_CP004887:c5880094-5879807 [Klebsiella michiganensis HKOPL1]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT45062 WP_014227757.1 NZ_CP004887:c5879820-5879518 [Klebsiella michiganensis HKOPL1]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT45062 NZ_CP004887:c5879820-5879518 [Klebsiella michiganensis HKOPL1]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591