Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5172410..5173029 Replicon chromosome
Accession NZ_CP004887
Organism Klebsiella michiganensis HKOPL1

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag J415_RS24045 Protein ID WP_004099646.1
Coordinates 5172811..5173029 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag J415_RS24040 Protein ID WP_004129911.1
Coordinates 5172410..5172784 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J415_RS24030 5167567..5168760 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J415_RS24035 5168783..5171929 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
J415_RS24040 5172410..5172784 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
J415_RS24045 5172811..5173029 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
J415_RS24050 5173192..5173758 + 567 WP_038423299.1 maltose O-acetyltransferase -
J415_RS24055 5173885..5174355 + 471 WP_014228205.1 YlaC family protein -
J415_RS24060 5174330..5175784 - 1455 WP_014228204.1 PLP-dependent aminotransferase family protein -
J415_RS24065 5175886..5176584 + 699 WP_014228203.1 GNAT family N-acetyltransferase -
J415_RS24070 5176581..5176721 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
J415_RS24075 5176721..5176984 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T45059 WP_004099646.1 NZ_CP004887:5172811-5173029 [Klebsiella michiganensis HKOPL1]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T45059 NZ_CP004887:5172811-5173029 [Klebsiella michiganensis HKOPL1]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT45059 WP_004129911.1 NZ_CP004887:5172410-5172784 [Klebsiella michiganensis HKOPL1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45059 NZ_CP004887:5172410-5172784 [Klebsiella michiganensis HKOPL1]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References