Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2732472..2733062 Replicon chromosome
Accession NZ_CP004887
Organism Klebsiella michiganensis HKOPL1

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag J415_RS12815 Protein ID WP_014229979.1
Coordinates 2732730..2733062 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag J415_RS12810 Protein ID WP_004852307.1
Coordinates 2732472..2732729 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J415_RS12785 2727728..2728336 - 609 WP_014229984.1 glutathione S-transferase family protein -
J415_RS12790 2728513..2729439 + 927 WP_014229983.1 LysR family transcriptional regulator -
J415_RS12795 2729477..2730475 - 999 WP_014229982.1 aldo/keto reductase -
J415_RS12800 2730576..2731490 + 915 WP_014229981.1 LysR family transcriptional regulator -
J415_RS29330 2731681..2731761 - 81 Protein_2520 molybdopterin-binding protein -
J415_RS12810 2732472..2732729 + 258 WP_004852307.1 antitoxin Antitoxin
J415_RS12815 2732730..2733062 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J415_RS12820 2733775..2734581 + 807 WP_064387424.1 hypothetical protein -
J415_RS29040 2734676..2735134 + 459 WP_051597161.1 hypothetical protein -
J415_RS29045 2735152..2735946 + 795 WP_125932918.1 hypothetical protein -
J415_RS29050 2736035..2737030 + 996 WP_051597163.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T45050 WP_014229979.1 NZ_CP004887:2732730-2733062 [Klebsiella michiganensis HKOPL1]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T45050 NZ_CP004887:2732730-2733062 [Klebsiella michiganensis HKOPL1]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT45050 WP_004852307.1 NZ_CP004887:2732472-2732729 [Klebsiella michiganensis HKOPL1]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT45050 NZ_CP004887:2732472-2732729 [Klebsiella michiganensis HKOPL1]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References