Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1569016..1569673 Replicon chromosome
Accession NZ_CP004887
Organism Klebsiella michiganensis HKOPL1

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag J415_RS07395 Protein ID WP_004854060.1
Coordinates 1569263..1569673 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag J415_RS07390 Protein ID WP_004124953.1
Coordinates 1569016..1569282 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J415_RS07375 1564249..1564674 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
J415_RS07380 1564795..1567593 - 2799 WP_014226794.1 transcriptional regulator DagR -
J415_RS07385 1567787..1568771 - 985 Protein_1442 tRNA-modifying protein YgfZ -
J415_RS07390 1569016..1569282 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
J415_RS07395 1569263..1569673 + 411 WP_004854060.1 protein YgfX Toxin
J415_RS07400 1569682..1570203 - 522 WP_014226792.1 flavodoxin FldB -
J415_RS07405 1570325..1571221 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
J415_RS07410 1571263..1571958 + 696 WP_038422976.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J415_RS07415 1571964..1573697 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T45049 WP_004854060.1 NZ_CP004887:1569263-1569673 [Klebsiella michiganensis HKOPL1]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T45049 NZ_CP004887:1569263-1569673 [Klebsiella michiganensis HKOPL1]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTTGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT45049 WP_004124953.1 NZ_CP004887:1569016-1569282 [Klebsiella michiganensis HKOPL1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT45049 NZ_CP004887:1569016-1569282 [Klebsiella michiganensis HKOPL1]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References