Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 763415..763962 Replicon chromosome
Accession NZ_CP004887
Organism Klebsiella michiganensis HKOPL1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag J415_RS03560 Protein ID WP_014227299.1
Coordinates 763415..763723 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag J415_RS03565 Protein ID WP_014227298.1
Coordinates 763726..763962 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J415_RS03530 759038..759349 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
J415_RS03535 759644..760585 + 942 WP_014227303.1 LacI family transcriptional regulator -
J415_RS03540 760609..760902 - 294 WP_014227302.1 hypothetical protein -
J415_RS03545 761112..762101 + 990 WP_014227301.1 Rpn family recombination-promoting nuclease/putative transposase -
J415_RS03550 762101..762358 + 258 WP_038422923.1 hypothetical protein -
J415_RS03555 762362..763264 - 903 WP_014227300.1 EamA family transporter -
J415_RS03560 763415..763723 - 309 WP_014227299.1 CcdB family protein Toxin
J415_RS03565 763726..763962 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J415_RS03570 764068..765501 - 1434 WP_014227297.1 PTS N-acetylmuramic acid transporter subunit IIBC -
J415_RS03575 765526..766428 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
J415_RS03580 766589..767755 - 1167 WP_014227296.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T45048 WP_014227299.1 NZ_CP004887:c763723-763415 [Klebsiella michiganensis HKOPL1]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T45048 NZ_CP004887:c763723-763415 [Klebsiella michiganensis HKOPL1]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT45048 WP_014227298.1 NZ_CP004887:c763962-763726 [Klebsiella michiganensis HKOPL1]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT45048 NZ_CP004887:c763962-763726 [Klebsiella michiganensis HKOPL1]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References